基于Nano-ICG增强巨噬细胞活体光声成像的急性呼吸道炎症无创定量评估  

Nondestructive Quantitative Assessment of Acute Airway Inflammation Based on Nano-ICG-Enhanced In Vivo Photoacoustic Imaging of Macrophages

作　　者：张建[1,2] 梁超豪 罗志佳 孟凡 张艺晴 王倩[1] Zhang Jian;Liang Chaohao;Luo Zhijia;Meng Fan;Zhang Yiqing;Wang Qian(Medical Imaging Innovation Laboratory,School of Biomedical Engineering,Guangzhou Medical University,Guangzhou 511436,Guangdong,China;State Key Laboratory of Respiratory Diseases,First Affiliated Hospital,Guangzhou Medical University,Guangzhou 510120,Guangdong,China)

机构地区：[1]广州医科大学生物医学工程学院医学影像创新实验室,广东广州511436 [2]广州医科大学附属第一医院呼吸疾病国家重点实验室,广东广州510120

出　　处：《中国激光》2024年第9期181-189,共9页Chinese Journal of Lasers

基　　金：国家重点研发计划(2022YFC2304205);广东省医学科学技术研究基金(B2020061);广东省中医药局科研项目(20232104);广州医科大学科研能力提升项目(02-410-2302327XM)。

摘　　要：巨噬细胞作为炎症阶段的主要吞噬细胞,其高表达是急性呼吸道炎症发展过程的临床特征之一。目前还没有一种成像方法能够以深组织穿透性和高分辨率的方式呈现巨噬细胞在急性炎症中的表达。以吲哚菁绿纳米颗粒(Nano-ICG)作为一种高效的光声成像(PAI)增强造影剂,评估了急性呼吸道炎症中巨噬细胞的表达量。激光共聚焦显微镜下的成像效果证实,Nano-ICG能够快速地被巨噬细胞吞噬。利用Nano-ICG增强光声成像效果后,气管内的PAI结果显示了巨噬细胞在炎症后气管壁上的分布区域。Nano-ICG增强的光声成像能够无创、定量地评估急性呼吸道炎症的发展程度,有望为呼吸疾病相关基础研究和临床诊疗提供新的影像技术支持。Objective Respiratory viruses possess strong infectivity,rapid transmission,short incubation periods,and sudden onset of illness.These features have led to widespread global transmission,significantly affecting the health of children worldwide.In addition,these viruses have caused significant economic losses and casualties in various countries.Antibiotics are commonly used to control respiratory infections in humans.Therefore,accurate and rapid understanding of the course of respiratory infections is the foundation for selecting a treatment plan.In biomedical imaging,various imaging methods can reveal microscopic and macroscopic phenomena within organisms.These methods include magnetic resonance imaging(MRI),computed tomography,positron emission tomography,ultrasound(US)imaging,optical coherence tomography,and fluorescence imaging.These technologies provide rich information,thereby contributing to a comprehensive understanding of the characteristics of respiratory infections and supporting the development of rational treatment plans.Owing to limitations in specificity,resolution,and radiation,these imaging techniques lack the ability to accurately image biological structures in the early stages of disease development.In this study,the noninvasive,deep-penetrating,and high spatial resolution advantages of photoacoustic(PA)imaging(PAI)are utilized.This is combined with the excellent fluorescence properties of the exogenous contrast agent indocyanine green nanoparticles(nano-ICG)in the near-infrared region and the high expression of macrophages during inflammation.This combination enables the visualization of the development of respiratory inflammation.Through the establishment of animal models and in vivo experiments,we quantitatively evaluate the macrophage expression in acute respiratory infections,as shown in Fig.1.Research on PAI is expected to provide a new approach for the noninvasive quantitative assessment of inflammation in acute respiratory infections.Methods This study uses a respiratory inflammation mou

关 键 词：医用光学 光声成像 急性呼吸道炎症 吲哚菁绿纳米颗粒 巨噬细胞 

分 类 号：O439[机械工程—光学工程]