基于FPGA的实时荧光相关光谱三元数字相关器的设计与实现  

Design and Implementation of Real-Time Fluorescence Triple Correlation Spectroscopy Correlator Based on FPGA

作　　者：孙亚楠 蔡萍[1] 王伟达 陆欣伟 董朝青[2] 任吉存[2] Sun Yanan;Cai Ping;Wang Weida;Lu Xinwei;Dong Chaoqing;Ren Jicun(Department of Instrument Science and Engineering,School of Electronic Information and Electrical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China;School of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学电子信息与电气工程学院仪器科学与工程系,上海200240 [2]上海交通大学化学化工学院,上海200240

出　　处：《激光与光电子学进展》2024年第9期419-427,共9页Laser & Optoelectronics Progress

基　　金：国家重大科研仪器研制项目(22027803)。

摘　　要：面向荧光相关光谱分析应用,研究基于现场可编程逻辑门阵列(FPGA)技术的多通道实时荧光相关光谱三元数字相关器的实现方法。硬件结构基于寄存器级硬件描述语言设计和实现。基于荧光相关光谱的特点,采用多采样时间相关和模块“分时复用”的设计思想,有效减少了运算量和硬件资源消耗。将二元相关的分时复用思想扩展至三元相关,提出了适用于三元相关的分时复用方法,实现了基于FPGA的实时计算的三元数字相关器。通过对称归一化的计算方法保证了相关函数的计算精度。光子相关器基于Xilinx Zynq-7100 FPGA芯片实现,实现了三路自相关、三路互相关和一路三元相关的实时计算。实验验证了相关计算的准确性,最高时间分辨率可达40 ns,动态范围为1.57×10^(7)。To meet the requirements of multichannel fluorescence correlation spectroscopy with a wide dynamic range,a digital correlator based on field programmable gate array(FPGA)technology was designed and implemented.The hardware structure was designed and implemented using a registerlevel hardware description language.Based on the characteristics of photon pulse counting signals,the multisamplingtime correlation and timedivision multiplexing methods were used to calculate the correlation function,which substantially improved computing efficiency and optimized hardware resource utilization.The timedivision multiplexing of cross correlation was extended to triple correlation,and the corresponding method was extended to adapt triple correlation.Ultimately,a realtime computing triple correlator based on FPGA was realized.The calculation method of symmetric normalization ensured the accuracy of the correlation function.The designed photon correlator was implemented based on a single Xilinx Zynq7100 FPGA chip,which performed various functions,including the realtime correlation of three channels autocorrelation,three channels crosscorrelation,and one channel triple correlation,with the time resolution of 40 ns and dynamic range of 1.57×10^(7).

关 键 词：荧光相关光谱 数字相关器 多采样时间相关法 光子脉冲计数 分时复用 

分 类 号：O657.3[理学—分析化学]