Mutation in a non-force-bearing region of protein L influences force-dependent unfolding behavior  

作　　者：蒋环杰 王艳伟 陈家媛 胡丹 潘海 郭子龙 陈虎 Huanjie Jiang;Yanwei Wang;Jiayuan Chen;Dan Hu;Hai Pan;Zilong Guo;Hu Chen(Department of Physics,Wenzhou University,Wenzhou 325035,China;Center of Biomedical Physics,Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325000,China;Research Institute for Biomimetics and Soft Matter,Fujian Provincial Key Laboratory for Soft Functional Materials Research,Department of Physics,Xiamen University,Xiamen 361005,China)

机构地区：[1]Department of Physics,Wenzhou University,Wenzhou 325035,China [2]Center of Biomedical Physics,Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325000,China [3]Research Institute for Biomimetics and Soft Matter,Fujian Provincial Key Laboratory for Soft Functional Materials Research,Department of Physics,Xiamen University,Xiamen 361005,China

出　　处：《Chinese Physics B》2024年第7期624-629,共6页中国物理B（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.12174322 to HC and 12204124 to ZG);111 Project(Grant No.B16029);the Graduate Scientific Research Foundation of Wenzhou University(Grant No.3162023003034 to JH);research grant from Wenzhou Institute。

摘　　要：Single-molecule magnetic tweezers(MTs) have revealed multiple transition barriers along the unfolding pathway of several two-state proteins, such as GB1 and Csp. In this study, we utilized MTs to measure the force-dependent folding and unfolding rates of both protein L(PLWT) and its Y47W mutant(PLY47W) where the mutation point is not at the force-bearing β-strands. The measurements were conducted within a force range of 3–120 pN. Notably, the unfolding rates of both PLWT and PWY47W exhibit distinct force sensitivities below 50 pN and above 60 pN, implying a two-barrier free energy landscape. Both PLWT and PLY47W share the same force-dependent folding rate and the same transition barriers,but the unfolding rate of PLY47W is faster than that of PLWT. Our finding demonstrates that the residue outside of the force-bearing region will also affect the force-induced unfolding dynamics.

关 键 词：protein folding magnetic tweezers protein L 

分 类 号：Q51[生物学—生物化学]