短小芽孢杆菌DST27发酵培养基及发酵条件的优化  

Optimization of fermentation medium and conditions for Bacillus pumilus DST27

作　　者：李铮 王艳 晋玲 王伟 付瑶 栾涵钰 吕娇娇 LI Zheng;WANG Yan;JIN Ling;WANG Wei;FU Yao;LUAN Hanyu;LYU Jiaojao(College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China;Northwest Collaborative Innovation Center for Traditional Chinese Medicine Co-constructed by Gansu Province&MOE of PRC,Lanzhou 730000,China;Research Center of Traditional Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学药学院,兰州730000 [2]西北中藏药省部共建协同创新中心,兰州730000 [3]甘肃省中医药研究中心,兰州730000

出　　处：《农药》2024年第7期500-506,514,共8页Agrochemicals

基　　金：西北中藏药省部共建协同创新中心开放基金项目(Xbzzy-2022-02);中药保障与创新能力提升项目(甘中医药综函[2024]14号);甘肃省科技计划项目(23ZDFA013-1);甘肃省2023年度优秀研究生“创新之星”(2023CXZX-763);甘肃省中医药研究中心开放课题(zyzx-2023-20)。

摘　　要：[目的]通过优化生防菌株短小芽孢杆菌DST27发酵培养基及发酵培养条件,为后期该菌株的开发和应用提供基础。[方法]通过单因素筛选、Plackett-Burman试验和响应面分析法,以菌体生长量和对尖孢镰刀菌的抑菌活性为指标,优化DST27发酵培养基及发酵培养条件。[结果]最佳发酵培养基为1.35%葡萄糖、1%酵母粉和0.5%CaCl2;最佳发酵条件为装液量20%、接种量5.40%、初始pH 7.28、温度28℃和转速180 r/min,在此培养条件下DST27的OD600为2.256,抑菌率为78.65%,与模型预测值81.42%相近,较优化前提高了22.06%。[结论]该试验优化了DST27发酵培养基及发酵条件,为今后该菌剂生产工艺及开展防治提供理论依据。[Aims]This study aims to establish a foundation for the later development and use of the biocontrol strain Bacillus pumilus DST27 by optimizing the fermentation medium and circumstances.[Methods]Based on single factor test,Plackett-Burman test and response surface analysis,the fermentation medium and conditions of DST27 were optimized with the bacterial biomass and the antifungal activity against Fusarium oxysporum as indicators.[Results]The optimal medium for liquid fermentation of strain DST27 was 1.35%glucose,1%yeast powder,and 0.5%CaCl.The optimal fermentation conditions were as follows:liquid-loading rate 20%,inoculation amount 3%,pH 7.28,temperature 28℃,time 60 h,and rotating speed 180 r/min.The ODoo of DST27 was 2.256 under these cultivation circumstances,and the antifungal rate was 78.65%,which was similar to the predicted value of 81.42%in the model,and had increased by 22.06%compared to that before optimization.[Conclusions]This experiment optimized the fermentation medium and conditions of DST27,providing a theoretical basis for the production process of this bacterial agent and prevention with it in the future.

关 键 词：短小芽孢杆菌DST27 培养基优化 发酵条件优化 PLACKETT-BURMAN设计 响应面分析法 

分 类 号：TQ450.2[化学工程—农药化工]