A亚型呼吸道合胞病毒感染患者血清中免疫相关分子的表达研究  

Expressions of immune-associated molecules in serum of patients infected with respiratory syncytial virus subtype A

作　　者：申宜 马倩格 李涛 谢广成[1] Shen Yi;Ma Qiange;Li Tao;Xie Guangcheng(Department of Pathogenic Biology,Chengde Medical University,Chengde 067000,China;No.2 Clinical Teaching Hospital of Chengde Medical University,Chengde 067000,China)

机构地区：[1]承德医学院病原生物学教研室,承德067000 [2]承德医学院第二临床教学医院,承德067000

出　　处：《中华实验和临床病毒学杂志》2024年第3期239-246,共8页Chinese Journal of Experimental and Clinical Virology

基　　金：国家重点研发计划(2022YFC3500800,2022YFC3500802);国家自然科学基金(81702008);河北省自然科学基金(H2018406024)。

摘　　要：目的探讨A亚型呼吸道合胞病毒(respiratory syncytial virus subtype A,RSV-A)感染患者血清中免疫相关分子的表达改变情况。方法2022年8月至2023年6月,收集在承德医学院第二临床教学医院儿科住院治疗的呼吸道感染患者的血清样本,根据患者呼吸道病原体检测结果分组为RSV-A单独感染和RSV-A共感染,使用酶联免疫吸附试验试剂盒检测Th1细胞因子干扰素γ(interferonγ,IFN-γ)和肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)、Th2细胞因子白介素4(interleukin 4,IL-4)和IL-10、可溶性Toll样受体2(soluble toll-like receptor 2,sTLR2)和4(sTLR4)、可溶性肿瘤坏死因子受体1(soluble tumor necrosis factor receptor 1,sTNFR1)和2(sTNFR2)。结果RSV-A单独感染和RSV-A共感染患者血清中IFN-γ(H=10.030,P=0.007)和IL-4(H=10.246,P=0.006)的表达水平显著提升,而TNF-α(F=1.154,P=0.322)和IL-10(F=1.738,P=0.188)的表达水平并未显著提高。IFN-γ和IL-4的曲线下面积(area under the curve,AUC)分别为0.866(95%CI:0.767~0.966,P=0.002),0.986(95%CI:0.956~1.000,P=0.001)。sTLR2(H=1.165,P=0.559)和sTLR4(H=1.657,P=0.437)的表达水平并未发生显著改变。sTNFR1(H=11.431,P=0.003)和sTNFR2(F=3.411,P=0.041)的表达水平在RSV-A共感染患者血清中显著提升且sTNFR2浓度高于sTNFR1,sTNFR1在RSV-A合并细菌感染和RSV-A合并双重细菌感染患者中血清中浓度最高。sTNFR2的AUC为0.967(95%CI:0.911~1.000,P=0.002)。结论RSV-A感染改变患者血清中免疫相关分子IFN-γ、IL-4、sTNFR1和sTNFR2的表达,上述免疫分子可能参与到RSV-A与宿主互作和感染致病过程。Objective To determine the changes of immune-associated molecules in serum of patients infected with respiratory syncytial virus subtype A(RSV-A).Methods Serum specimens were collected from patients with respiratory infection at Department of Pediatrics of NO.2 Clinical Teaching Hospital of Chengde Medical University during August 2022 to June 2023.The serum specimens were set as RSV-A single infection group and RSV-A coinfection group based on the detected pathogens in these patients.Serum levels of Th1 cytokines including interferonγ(IFN-γ)and tumor necrosis factor-α(TNF-α),and Th2 cytokines including interleukin 4(IL-4)and IL-10,soluble toll-like receptor 2(sTLR2)and 4(sTLR4),soluble tumor necrosis factor receptor 1(sTNFR1)and sTNFR2 were determined using enzyme-linked immunosorbent assay(ELISA)kits.Results Serum levels of IFN-γ(H=10.030,P=0.007)and IL-4(H=10.246,P=0.006)were significantly up-regulated in RSV-A single infection group and RSV-A coinfection group,however,levels of TNF-α(F=1.154,P=0.322)and IL-10(F=1.738,P=0.188)were not significantly up-regulated.Area under the curve(AUC)of IFN-γand IL-4 were 0.866(95%CI:0.767-0.966,P=0.002)and 0.986(95%CI:0.956-1.000,P=0.001),respectively.The levels of sTLR2(H=1.165,P=0.559)and sTLR4(H=1.657,P=0.437)were not significantly changed.Levels of sTNFR1(H=11.431,P=0.003)and sTNFR2(F=3.411,P=0.041)were significantly up-regulated in RSV-A coinfection group and the concentration of sTNFR2 was higher than sTNFR1.Concentration of sTNFR1 was peaked in RSV-A coinfected with bacterium group and RSV-A coinfected with dual bacteria group.AUC of sTNFR2 was 0.967(95%CI:0.911-1.000,P=0.002).Conclusions Expressions of immune-associated molecules,including IFN-γ,IL-4,sTNFR1 and sTNFR2 were significantly changed in patients infected with RSV-A,and these immune-associated molecules maybe engage in the interaction and pathogenesis between RSV-A and host.

关 键 词：A亚型呼吸道合胞病毒 细胞因子 可溶性Toll样受体 可溶性肿瘤坏死因子受体 

分 类 号：R56[医药卫生—呼吸系统]