微波对模拟感染性废物中腺病毒衣壳完整性及Hexon蛋白抗原性的影响  

Effect of microwave on adenovirus capsid integrity and antigenicity of hexon protein in simulated infectious wastes

作　　者：王扩 丁淑军[2] 李丹迪 杨晓斌 秦泽明 赵丽 唐浏英[3] Wang Kuo;Ding Shujun;Li Dandi;Yang Xiaobin;Qin Zeming;Zhao Li;Tang Liuying(Department of Microbiological Laboratory Technology,School of Public Health,Cheeloo College of Medicine,Key Laboratory of Infectious Disease Control and Prevention in Universities of Shandong,Shandong University,Jinan 250012,China;Shandong Center for Disease Control and Prevention,Shandong Provincial Key Laboratory of Infectious Disease Prevention and Control,Jinan 250014,China;National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China)

机构地区：[1]山东大学齐鲁医学院公共卫生学院微生物检验学系,山东省"十三五"高等学校感染性疾病防控重点实验室,济南250012 [2]山东省疾病预防控制中心,山东省传染病预防控制重点实验室,济南250014 [3]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《中华实验和临床病毒学杂志》2024年第3期247-251,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的研究微波对模拟感染性废物中人腺病毒2型(human adenovirus,HAdV-2)衣壳蛋白的影响。方法将HAdV-2病毒悬液滴加至医用一次性手套模拟感染性废物,在不同微波条件下对其进行辐照,记录温度变化,辐照后的病毒上清液经Dnase I处理后通过PCR、qPCR检测病毒核酸,判断微波对病毒衣壳完整性的影响。ELISA检测微波辐照对病毒Hexon蛋白结构的影响。单独以微波辐照过程中的最高温度处理病毒,研究微波消毒过程是否存在非热效应。结果微波辐照过程中最高温度为76℃,PCR及qPCR结果表明,Dnase I可使微波辐照后的病毒核酸产生明显损伤,而病毒对照组及热处理组没有受到明显影响,表明微波辐照可以破坏病毒衣壳完整性。ELISA结果表明,微波辐照能使病毒Hexon蛋白与抗体结合能力明显减弱,热处理组结果与之相似。结论微波辐照可破坏HAdV-2衣壳完整性以及Hexon蛋白结构,其中衣壳完整性受损主要是非热效应发挥作用,Hexon蛋白结构改变主要是热效应发挥作用。Objective To investigate the effect of microwave on human adenovirus type 2(HAdV-2)capsid protein in simulated infectious wastes.Methods Droplets of HAdV-2 virus suspension were added to medical disposable gloves to simulate infectious waste,irradiated under different microwave conditions,the temperature change was recorded,and the irradiated viral supernatant was treated with Dnase I and detected by PCR and qPCR to determine the effect of microwave on the integrity of the viral capsid.ELISA was used to detect the effect of microwave irradiation on the structure of viral hexon protein.The virus was treated alone at the highest temperature during microwave irradiation to investigate whether there were non-thermal effects during microwave disinfection.Results The maximum temperature during microwave irradiation was 76℃,and the PCR and qPCR result showed that Dnase I could significantly damage the viral nucleic acid after microwave irradiation,while the virus control group and heat treatment group were not significantly affected,indicating that microwave irradiation could destroy the integrity of the viral capsid.The result of ELISA showed that microwave irradiation could significantly weaken the binding ability of Hexon protein and antibody,and the result of heat treatment group were similar.Conclusions Microwave irradiation can destroy the integrity of the HAdV-2 capsid and the structure of Hexon protein,in which the damage to the integrity of the capsid is mainly due to non-thermal effects,and the structural changes of hexon protein are mainly due to thermal effects.

关 键 词：微波 感染性废物 腺病毒 衣壳 灭活机制 

分 类 号：R511.8[医药卫生—内科学]