COPD急性加重期并发呼吸道病毒感染患者血清及肺泡灌洗液中CCL2和POSTN水平与肺功能的相关性分析  

Correlation analysis between CCL 2 and POSTN levels and lung function in serum and alveolar lavage fluid of patients with respiratory virus infection during acute exacerbation of COPD

作　　者：田刚 林祥晓[1] 陈莉 胡依粉 崔芹 蔡静静 宋敏[1] 齐洁[2] Tian Gang;Lin Xiangxiao;Chen Li;Hu Yifen;Cui Qin;Cai Jingjing;Song Min;Qi Jie(Department of Respiratory and Critical Care Medicine,Affiliated Hospital of Jining Medical University,Jining 272029,China;Tracheoscopy Laboratory,Affiliated Hospital of Jining Medical University,Jining 272029,China)

机构地区：[1]济宁医学院附属医院呼吸与危重症医学科,济宁272029 [2]济宁医学院附属医院气管镜室,济宁272029

出　　处：《中华实验和临床病毒学杂志》2024年第3期258-262,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：山东省医药卫生科技发展计划(202211000699)。

摘　　要：目的探究慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)急性加重期并发呼吸道病毒感染患者血清及肺泡灌洗液中CC趋化因子配体2(CC chemokine ligand 2,CCL2)、骨膜蛋白(Periostin,POSTN)水平与肺功能的相关性。方法选取2020年3月至2023年3月本院收治的COPD急性加重期患者96例,其中并发呼吸道病毒感染患者34例即为感染组,未发生呼吸道病毒感染62例即为未感染组。采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)法检测血清及肺泡灌洗液中CCL2、POSTN的表达水平。Pearson法分析感染组患者血清及肺泡灌洗液中CCL2、POSTN水平与肺功能指标的相关性。结果与未感染组相比,感染组患者血清及肺泡灌洗液中CCL2(t=12.633、9.252)、POSTN(t=12.370、7.383)水平均显著升高(P<0.05)。与未感染组患者相比,感染组患者肺功能指标6 min步行试验(six-minute walk test,6MWT)、呼气峰值流速(peak expiratory flow,PEF)、第1 sec用力呼气量(forced expiratory volume in one second,FEV1)、用力肺活量(forced vital capacity,FVC)、FEV1/FVC、最大呼气中段平均流速(maximal mid-expiratory flow curve,MMEF)水平显著降低(t=14.141、24.165、22.421、21.223、5.278、29.456,P<0.05)。相关性分析显示,感染组患者血清及肺泡灌洗液中CCL2、POSTN水平与6MWT、PEF、FEV1、FVC、FEV1/FVC、MMEF水平呈负相关关系(P<0.05)。结论COPD急性加重期并发呼吸道病毒感染患者血清及肺泡灌洗液中CCL2、POSTN水平呈高表达,二者与肺功能密切相关。Objective To investigate the correlation between the levels of CC chemokine ligand 2(CCL2)and periostin(POSTN)in serum and alveolar lavage fluid of patients with acute exacerbation of chronic obstructive pulmonary disease(COPD)complicated with respiratory virus infection and lung function.Methods From March 2020 to March 2023,96 patients with acute exacerbation of COPD admitted to our hospital were collected.Among them,34 patients with concurrent respiratory virus infection were included in the infected group,and 62 patients without respiratory virus infection were included in the uninfected group.Enzyme linked immunosorbent assay(ELISA)was applied to detect the expression levels of CCL2 and POSTN in serum and alveolar lavage fluid.Pearson method was applied to analyze the correlation between CCL2 and POSTN levels in serum and alveolar lavage fluid of infected patients and lung function indicators.Results The levels of CCL 2(t=12.633,9.2532,2)and POSTN(t=12.370,7.383)were significantly increased in the infected group compared with the uninfected group(P<0.05).Compared with the uninfected group,the 6-minute walking test(6 MWT),peak expiratory flow rate(peak expiratory flow,PEF),forced expiratory volume at the first second(forced expiratory volume in one second,FEV 1),and the forced lung capacity(forced vital capacity,FVC),FEV 1/FVC,and maximum middle breath mean flow rate(maximal mid-expiratory flow curve,MMEF)were significantly lower(t=14.141,24.165,22.421,21.223,5.278,29.456,P<0.05).Correlation analysis showed that the levels of CCL2 and POSTN in the serum and alveolar lavage fluid of the infected group were negatively correlated with the levels of 6MWT,PEF,FEV1,FVC,FEV1/FVC,and MMEF(P<0.05).Conclusions CCL2 and POSTN levels were highly expressed in serum and alveolar lavage fluid of patients with respiratory virus infection during acute exacerbation of COPD,which were closely related to lung function.

关 键 词：慢性阻塞性肺病 呼吸道病毒感染 CC趋化因子配体2 骨膜蛋白 肺功能 

分 类 号：R563.9[医药卫生—呼吸系统]