2021-2022年杭州新型冠状病毒阳性病例中人鼻病毒的共感染特征分析  

Characteristics of human rhinovirus co-infections observed in 2019-nCoV positive patients in Hangzhou from 2021 to 2022

作　　者：程实 于新芬[1] 曹飞飞 周银燕[1] 潘劲草[1] Cheng Shi;Yu Xinfen;Cao Feifei;Zhou Yinyan;Pan Jincao(Health Inspection Center,Hangzhou Center for Disease Control and Prevention(Hangzhou Health Supervision Institution),Hangzhou 310021,China)

机构地区：[1]杭州市疾病预防控制中心(杭州市卫生监督所)卫生检验中心,杭州310021

出　　处：《中华实验和临床病毒学杂志》2024年第3期299-303,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：浙江省医药卫生科技计划(2020KY237);杭州市医药卫生科技项目(A20200487)。

摘　　要：目的了解新型冠状病毒(2019 novel coronavirus,2019-nCoV)阳性病例中人鼻病毒(human rhinovirus,HRV)的共感染特征及系统进化特征。方法使用实时荧光定量PCR(real-time fluorescence quantitative polymerase chain reaction,qPCR)对7213份2019-nCoV核酸阳性样本进行了包括HRV在内的10种常见呼吸道病毒检测,分析其共感染特征;对HRV核酸阳性样本进行了VP4/VP2基因片段测序并构建进化树,分析其系统进化特征。结果2019-nCoV核酸阳性样本中合并HRV感染最多,为97例,阳性率1.34%;其次是普通冠状病毒(coronavirus,CoV),为36例,阳性率0.50%。HRV共感染率与其他病毒相比,差异具有统计学意义(χ^(2)=318.09,P<0.001)。18岁以下组HRV共感染率最高,差异具有统计学意义(χ^(2)=36.77,P<0.001)。共感染HRV未呈现明显季节性。其中39株HRV基因片段测序成功(39/97,40.21%),进化树分析表明,10株被划分到HRV-A组,9株被划分到HRV-B组,20株被划分到HRV-C组。39份样本共检测到17个血清型,其中B6和C15为优势血清型,分别为6株(HRV-B中占比66.67%)和14株(HRV-C中占比70%),其他血清型呈散在分布。结论HRV是2019-nCoV核酸阳性病例中共感染率最高的病毒类呼吸道病原体,共感染率在18岁以下组最高。杭州地区2019-nCoV阳性病例中共感染的HRV-A组、HRV-B组和HRV-C组均有发现,血清型呈现多样性。Objective To investigate the co-infection and phylogenetic analysis of human rhinovirus(HRV)in 2019 novel coronavirus(2019-nCoV)positive samples.Methods Ten common respiratory viruses,including HRV were detected by real-time fluorescence quantitative polymerase chain reaction(qPCR)in 7213 samples of 2019-nCoV positive cases and the co-infection characteristics were analyzed.The VP4/VP2 gene fragment of HRV was amplified and sequenced.Phylogenetic trees were constructed.Results HRV accounted for 1.34%of the 2019-nCoV positive samples(97/7213),followed by common coronavirus(0.50%,36/7213).The co-infection rate of HRV in 2019-nCoV positive samples was significantly different from that of other viruses(χ^(2)=318.09,P<0.001).There was significant difference in HRV co-infection rate among different age groups(χ^(2)=36.77,P<0.001),the peak was in<18 years age group.The co-infection rate of HRV had no significant difference in different seasons.The VP4/VP2 gene fragments of 39 HRV strains(40.21%,39/97)were successfully sequenced and made phylogenetic analysis.There were 10 strains of HRV-A,9 strains of HRV-B and 20 strains of HRV-C.Seventeen subtypes were identified,of which B6(66.67%,6/9)and C15(70%,14/20)were the most prevalent and other subtypes were scattered.Conclusions The co-infection rate of HRV in patients with 2019-nCoV infection was the highest.The highest co-infection rate was in<18 years age group.Group A,B,and C of HRV were found in 2019-nCoV positive samples,and serotypes present diversity.

关 键 词：新型冠状病毒 人鼻病毒 共感染 

分 类 号：R511[医药卫生—内科学] R181.3[医药卫生—临床医学]