藜麦水溶蛋白的提取工艺优化及其酶解多肽抗氧化活性研究  

Optimization of Chenopodium quinoa Willd water-soluble protein extraction process and antioxidant activity of the enzymatic peptides

作　　者：褚明娟 赵蕙新 尚鹤婷 霍俊奇 武天煜 栗慧 王莹[1] 冯亭亭[1,2] 魏东 CHU Ming-Juan;ZHAO Hui-Xin;SHANG He-Ting;HUO Jun-Qi;WU Tian-Yu;LI Hui;WANG Ying;FENG Ting-Ting;WEI Dong(College of Traditional Chinese Medicine,Hebei North University,Zhangjiakou 075000,China;Hebei Key Laboratory of Analysis and Testing for Quality and Safety of Agricultural Products and Food,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院中医学院,张家口075000 [2]河北北方学院河北省农产品食品质量安全分析检测重点实验室,张家口075000

出　　处：《食品安全质量检测学报》2024年第11期301-309,共9页Journal of Food Safety and Quality

基　　金：张家口市市级科技计划自筹经费项目(2221005A);河北北方学院博士科研基金项目(BSJJ202317);河北省农产品食品质量安全分析检测重点实验室绩效补助经费项目(22567613H);河北北方学院基本科研业务费科学技术研究项目(JYT202204);河北北方学院校级科研项目(XJ2023041);河北省教育厅青年拔尖项目(BJS2024102)。

摘　　要：目的优化复合酶协同超声辅助法提取藜麦水溶蛋白的工艺,探讨7种商业蛋白酶对藜麦多肽的抗氧化活性的影响。方法采用Box-Behnken响应面法,对超声辅助糖化酶-纤维素酶复合酶法提取藜麦水溶蛋白的工艺进行优化。使用碱性蛋白酶、菠萝蛋白酶、风味蛋白酶、木瓜蛋白酶、酸性蛋白酶、胰蛋白酶和胃蛋白酶制备藜麦多肽,通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法和2,2’-联氮二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt,ABTS]法对其抗氧化活性进行探究。结果通过响应面模型对复合酶超声辅助提取条件进行优化,得到最佳提取条件为:总加酶量400 U/g、pH 5.0、时间60 min。在此条件下,藜麦水溶蛋白的提取率为75.09 mg/g。藜麦水溶蛋白在木瓜蛋白酶酶解4 h获得的藜麦多肽抗氧化活性最佳,DPPH自由基和ABTS阳离子自由基清除率分别为79.64%和76.14%。结论采用响应面法优化复合酶辅助超声法提取藜麦蛋白的工艺优化方法可行;藜麦蛋白水解物具有较好的抗氧化活性,为藜麦作为功能食品深入开发提供依据。Objective To optimize the extraction technology of Chenopodium quinoa Willd water-soluble protein with complex enzyme and ultrasonic assisted extraction and to study the antioxidant activity of Chenopodium quinoa Willd peptides.Methods The Box-Behnken response surface method was used to optimize the process of extracting water-soluble proteins from Chenopodium quinoa Willd using an ultrasonic-assisted enzymatic-cellulase complex method.Seven commercial proteases including alkaline protease,bromelain,flavourzyme,papain,acid protease,trypsin,and pepsin were used to prepare Chenopodium quinoa Willd peptides.The antioxidant activities of the peptides were investigated using the 1,1-diphenyl-2-picrylhydrazyl(DPPH)method and the 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)ammonium salt(ABTS)method.Results The response surface model was used to optimize the extraction conditions for the complex enzyme-assisted ultrasonic extraction,and the optimal extraction conditions were found to be a total enzyme addition of 400 U/g,pH 5.0,and a time of 60 min.Under these conditions,the extraction rate of Chenopodium quinoa Willd water-soluble protein was 75.09 mg/g.The Chenopodium quinoa Willd peptides obtained from papain hydrolysis for 4 h exhibited the highest antioxidant activity,with DPPH and ABTS radical scavenging rates of 79.64%and 76.14%respectively.Conclusion The process optimization method of using response surface methodology to optimize the composite enzyme assisted ultrasound extraction of Chenopodium quinoa Willd protein is feasible;Chenopodium quinoa Willd protein hydrolysates have good antioxidant activity,providing a basis for the in-depth development of quinoa as a functional food.

关 键 词：藜麦 水溶蛋白 响应面法 多肽 抗氧化活性 

分 类 号：TS210.4[轻工技术与工程—粮食、油脂及植物蛋白工程]