贵州省2022-2023年流感季A(H1N1)pdm09亚型流感病毒抗原性和血凝素基因特征  

Antigenicity and hemagglutinin gene characteristics of A(H1N1)pdm09 influenza viruses that circulated in Guizhou province during the 2022-2023 influenza season

作　　者：庄丽[1] 柯倩 郑菊 韦小瑜[1] Zhuang Li;Ke Qian;Zheng Ju;Wei Xiaoyu(Guizhou Provincial Center for Disease Control and Prevention,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004

出　　处：《中国疫苗和免疫》2024年第3期278-282,共5页Chinese Journal of Vaccines and Immunization

基　　金：贵州省传染病预防与控制人才基地科研团队(RCJD2104);贵州省科技计划项目(黔科合支撑[2021]一般025)。

摘　　要：目的了解贵州省A(H1N1)pdm09亚型流感病毒抗原性和血凝素(Hemagglutinin,HA)基因特征。方法选取贵州省流感监测点2022年10月至2023年3月A(H1N1)pdm09亚型流感病毒分离株,采用红细胞凝集抑制试验检测抗原性,测定HA全基因组序列,构建遗传进化树,分析核苷酸同源性和氨基酸位点变异。结果共获得78株A(H1N1)pdm09亚型流感病毒,均鉴定为疫苗类似株。22株毒株获得HA全基因组序列,与疫苗株的核苷酸同源性为97.28%-99.14%;其中6B.1A.5a.2a、6B.1A.5a进化分支分别为19株、3株;19株发生Sb抗原决定簇的A186T和Q189E氨基酸替换并分布在1个抗原决定簇,3株发生Sa抗原决定簇的K156N和I161L氨基酸替换并分布在1个抗原决定簇。19株发生190位螺旋(184-191)A186T和Q189E以及220位环E224A氨基酸替换,3株发生220位环D222Y氨基酸替换,均发生在HA1区域。未发现糖基化位点变异。结论贵州省2022-2023年流感季A(H1N1)pdm09亚型流感病毒与疫苗株匹配性良好,但存在部分氨基酸位点变异。需加强流感病毒监测及其抗原性和基因序列分析,以利于流感防控策略制定和流感疫苗株筛选。Objective To explore antigenicity and characteristics of hemagglutinin(HA)genes from A(H1N1)pdm09 influenza viruses that circulated in Guizhou province.Methods We obtained A(H1N1)pdm09 influenza viruses isolated from patients during October 2022 to March 2023 at influenza monitoring points of Guizhou.We assessed antigenicity using the red blood cell agglutination inhibition test,sequenced entire HA genomes and constructed a phylogenetic tree,and analyzed nucleotide homology and amino acid variation.Results A total of 78 A(H1N1)pdm09 influenza viruses were obtained and identified as vaccine-like strains.Entire HA genome sequences of 22 viruses had nucleotide homologies of 97.28%-99.14%compared with the vaccine strain;19 and 3 strains belonged to 6B.1A.5a.2a and 6B.1A.5a phylogenetic branches,respectively.Nineteen strains had A186T and Q189E amino acid substitutions in the Sb antigenic determinant of one determinant cluster and 3 strains had K156N and I161L substitutions in the Sa antigenic determinants of one determinant cluster.Nineteen strains had A186T and Q189E substitutions at the helix 190(184-191)and E224A substitution at the ring 220 and 3 strains had D222Y substitution at the ring 220 in the HA1 region.No glycosylation site variation was found.Conclusions The A(H1N1)pdm09 influenza virus isolated in Guizhou in the 2022-2023 influenza season matched with vaccine strains,but with some amino acid variation.We should strengthen monitoring and analysis of antigenicity and gene sequences of influenza viruses to optimize influenza prevention and control strategies and screen for vaccine strains.

关 键 词：A(H1N1)pdm09亚型流感病毒 抗原性 血凝素 基因特征 

分 类 号：R511.7[医药卫生—内科学]