不同月龄豚鼠自发膝骨关节炎过程中全膝关节的病理变化  

Pathological changes in the total knee joint during spontaneous knee osteoarthritis in guinea pigs at different months of age

作　　者：胡笑燊 李惠菁 吕君玲 肖显俊 李涓 李享[3] 刘凌 金荣疆 Hu Xiaoshen;Li Huijing;Lyu Junling;Xiao Xianjun;Li Juan;Li Xiang;Liu Ling;Jin Rongjiang(School of Health Preservation and Rehabilitation,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan Province,China;School of Clinical Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan Province,China;School of Acupuncture and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,Sichuan Province,China;Bayi Orthopedic Hospital,Chengdu 610075,Sichuan Province,China)

机构地区：[1]成都中医药大学养生康复学院,四川省成都市610075 [2]成都中医药大学临床医学院,四川省成都市610075 [3]成都中医药大学针灸推拿学院,四川省成都市610075 [4]八一骨科医院,四川省成都市610075

出　　处：《中国组织工程研究》2025年第11期2218-2224,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(82205239),项目负责人:胡笑燊;国家自然科学基金项目(82105027),项目负责人:李享。

摘　　要：背景:豚鼠因具有与人类相似的膝关节结构和接近的组织病理学特征,被认为是最有助于评估人类原发性骨关节炎的自发模型。目的:通过对1-18月龄豚鼠的全膝关节组织病理学分析,探讨豚鼠自发膝骨关节炎的病理过程。方法:选取健康1,6,10,14,16,18月龄Hartley雌性豚鼠各8只,将股四头肌进行苏木精-伊红染色,整体膝关节进行苏木精-伊红染色和甲苯胺蓝染色,在光镜下观察软骨、软骨下骨、滑膜、半月板、肌肉的组织病理学情况,进行关节软骨Mankin评分和滑膜炎评分,并进行二者的相关性分析。结果与结论:(1)随着豚鼠月龄的增长,关节软骨Mankin评分越高(P<0.05),滑膜炎病理评分也逐渐增高(P<0.05),且二者之间存在显著正相关(r=0.641,P<0.001);(2)18月龄豚鼠软骨下骨骨髓病变发病率达50%,半月板损伤发生率达37.5%;且出现不同程度的骨赘和关节间隙变窄等病变,仅有少数豚鼠出现股四头肌肉炎症;(3)结果说明豚鼠随着年龄增长,出现明显的软骨缺损、滑膜炎症、软骨下骨病变、半月板损伤、骨赘形成和关节间隙变窄等,均与人类原发性膝骨关节炎的病理过程相似,是理想的自发性膝骨关节炎模型。BACKGROUND:The guinea pig is considered to be the most useful spontaneous model for evaluating primary osteoarthritis in humans because of its similar knee joint structure and close histopathologic features to those of humans.OBJECTIVE:To investigate the pathological process of spontaneous knee osteoarthritis in guinea pigs by analyzing the histopathology of the total knee joint of guinea pigs aged 1 to 18 months.METHODS:Eight healthy female Hartley guinea pigs in each age group of 1,6,10,14,16,and 18 months old were selected.The quadriceps femoris was taken for hematoxylin-eosin staining,and the total knee joint was stained with hematoxylin-eosin and toluidine blue.The histopathology of the cartilage,subchondral bone,synovium,meniscus,and muscles were observed under light microscope.Mankin’s score and synovitis score were compared,and the correlation analysis was conducted.RESULTS AND CONCLUSION:As the guinea pig age increased,the Mankin’s score increased(P<0.05),and the pathological score of synovitis also gradually increased(P<0.05),and there was a significant positive correlation between the two(r=0.641,P<0.001).The incidence rate of subchondral bone marrow lesion in 18-month-old guinea pigs was 50%,and the incidence of meniscus injury was 37.5%.In addition,osteophyte and narrowing of the joint space were observed,and only a few guinea pigs had inflammation in the quadriceps femoris.To conclude,guinea pigs develop significant cartilage defects,synovial inflammation,subchondral bone lesions,meniscus injury,osteophyte formation,and joint space narrowing as they age,all of which are similar to the pathological processes of primary knee osteoarthritis in humans,making it an ideal model of spontaneous knee osteoarthritis.

关 键 词：膝骨关节炎 豚鼠 病理 软骨损伤 滑膜炎 骨髓损伤 

分 类 号：R446[医药卫生—诊断学] R318[医药卫生—临床医学] R496