阿仑膦酸钠干预去卵巢大鼠骨质疏松的腰椎代谢组学分析  

Metabolomics analysis of the lumbar spine after alendronate sodium intervention in ovariectomized rats with osteoporosis

作　　者：陈鑫飞 戴雅惠 谢冰颖 黄小彬 黄惠敏 黄景文 李生强 葛继荣 Chen Xinfei;Dai Yahui;Xie Bingying;Huang Xiaobin;Huang Huimin;Huang Jingwen;Li Shengqiang;Ge Jirong(Fujian University of Traditional Chinese Medicine,Fuzhou 350003,Fujian Province,China;Institute of Basic Research,Fujian Academy of Chinese Medical Science,Fuzhou 350003,Fujian Province,China;Fujian Key Laboratory of Combined Traditional Chinese and Western Medicines for Prevention and Treatment of Osteoporosis(Rehabilitation Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian Academy of Chinese Medical Sciences),Fuzhou 350003,Fujian Province,China)

机构地区：[1]福建中医药大学,福建省福州市350003 [2]福建省中医药科学院基础研究所,福建省福州市350003 [3]福建省中西医结合防治骨质疏松重点实验室(福建省中医药科学院,福建中医药大学附属康复医院),福建省福州市350003

出　　处：《中国组织工程研究》2025年第11期2277-2284,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81774350),项目负责人:李生强;福建省科技厅省属公益类科研院所基本科研专项(2019R1003-1),项目负责人:李生强;福建省自然科学基金项目(2023J01856),项目负责人:谢冰颖;福建省中医药科研项目(2021zylc42),项目负责人:李生强。

摘　　要：背景:研究报道,阿仑膦酸钠的摄入能显著提高骨质疏松症患者的骨密度。目的:利用色谱-质谱技术分析比对阿仑膦酸钠干预去卵巢大鼠模型前后代谢物变化,进一步探索阿仑膦酸钠治疗骨质疏松症的具体机制及作用靶点。方法:选择雌性SD大鼠36只,随机分为骨质疏松症组、阿仑膦酸钠组及假手术组,前两组采用去卵巢法建立骨质疏松症模型。造模4周后,阿仑膦酸钠组大鼠给予阿仑膦酸钠灌胃;假手术组和骨质疏松症组分别给予等体积生理盐水灌胃。连续灌胃12周后,通过色谱-质谱技术分析腰椎代谢物,得出共同差异代谢物,对其进行京都基因与基因组百科全书通路等生物信息学分析。结果与结论:①3组共得到17种共同显著性差异代谢物;②通过京都基因与基因组百科全书富集分析表明,阿仑膦酸钠可能调节不饱和脂肪酸生物合成、亚油酸代谢等通路保护去卵巢模型大鼠;③结果提示,阿仑膦酸钠可能通过干预不饱和脂肪酸生物合成代谢和亚油酸代谢发挥其抗骨质疏松作用,从而达到预防骨质疏松症的目的。BACKGROUND:Studies have reported that alendronate intake significantly increases bone mineral density in patients with osteoporosis.OBJECTIVE:To analyze and compare the changes in metabolites before and after alendronate intervention in ovariectomized rats by chromatography-mass spectrometry,and to further explore the specific mechanism and target of alendronate in the treatment of osteoporosis.METHODS:A total of 36 female Sprague-Dawley rats were randomly divided into model group,alendronate sodium group and sham operation group.The osteoporosis model was established by ovariectomy in the first two groups.Four weeks after modeling,the rats in the alendronate group were intragastrically given alendronate sodium,while those in the sham operation group and model group were given equal volume of normal saline.After 12 weeks of continuous gavage,the metabolites of the lumbar spine were analyzed by chromatography-mass spectrometry,and the common differential metabolites were obtained,which were analyzed by bioinformatics such as Kyoto Gene and Genome Encyclopedia pathway.RESULTS AND CONCLUSION:Totally 17 different metabolites were obtained in the three groups.The enrichment analysis of the Kyoto Encyclopedia of Genes and Genomes showed that alendronate sodium could regulate unsaturated fatty acid biosynthesis,linoleic acid metabolism and other pathways to protect ovariectomized rats.These results indicate that alendronate sodium may exert its anti-osteoporosis effect by interfering with unsaturated fatty acid bioanabolism and linoleic acid metabolism,so as to achieve the purpose of preventing osteoporosis.

关 键 词：骨质疏松 代谢组学 阿仑膦酸钠 亚油酸代谢 不饱和脂肪酸生物合成 差异代谢物 色谱-质谱技术 中医药 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R446