大麻二酚干预下力竭运动大鼠骨骼肌炎症相关基因的挖掘与验证  

Mining and verification of inflammation-related genes in skeletal muscle of exhaustive exercise rats undergoing cannabidiol intervention

作　　者：朱文宁 孙莉莉 彭丽娜 司俊成 臧万里 殷伟东 李孟琪 Zhu Wenning;Sun Lili;Peng Lina;Si Juncheng;Zang Wanli;Yin Weidong;Li Mengqi(Graduate School,Harbin Sport University,Harbin 150008,Heilongjiang Province,China;School of Human Sports Science,Harbin Sport University,Harbin 150008,Heilongjiang Province,China)

机构地区：[1]哈尔滨体育学院研究生院,黑龙江省哈尔滨市150008 [2]哈尔滨体育学院运动人体科学学院,黑龙江省哈尔滨市150008

出　　处：《中国组织工程研究》2025年第11期2347-2356,共10页Chinese Journal of Tissue Engineering Research

基　　金：黑龙江省省属本科高校基本科研业务费优秀青年创新团队项目(2023KYYWF-TD04),项目负责人:孙莉莉;哈尔滨体育学院引进人才科研启动费(RC20-202107),项目负责人:彭丽娜。

摘　　要：背景:大麻二酚能够有效改善机体的炎症反应,但在改善力竭运动引起的骨骼肌炎症方面,并未有明确的机制研究。目的:利用转录组学测序技术探究大麻二酚在力竭运动过程中对大鼠骨骼肌炎症的影响机制。方法:随机将36只SD大鼠分为6组:空白对照组、运动椰子油组、运动对照组、50 mg/kg大麻二酚组、60 mg/kg大麻二酚组、70 mg/kg大麻二酚组,每组6只。除空白对照组大鼠外,其余各组大鼠进行为期9 d的游泳运动制作力竭运动模型。每次游泳运动结束后,大麻二酚组大鼠给予不同浓度(50,60,70 mg/kg)脂溶性大麻二酚2 mL灌胃;运动椰子油组大鼠给予同等体积椰子油灌胃,直至第9天运动结束;空白对照组和运动对照组大鼠不做特殊处理。使用ELISA及转录组测序技术测定不同组大鼠骨骼肌中炎症因子的水平及差异基因,将得到的差异基因进行KEGG分析,并通过荧光定量PCR验证测序数据的准确性。结果与结论:(1)ELISA检测结果显示,相比于空白对照组和运动椰子油组,运动对照组中白细胞介素6(P<0.05)、肿瘤坏死因子α(P<0.01)、白细胞介素10等质量浓度均增加;经大麻二酚干预后,白细胞介素6、肿瘤坏死因子α质量浓度随大麻二酚浓度的加大而呈现依次递减的趋势;(2)通过GO和KEGG数据库的比较,对差异基因的功能特性进行分析,结果表明差异基因主要参与肿瘤坏死因子信号通路和Toll样受体信号通路;(3)RT-qPCR验证结果显示,随机选择5个差异基因的变化趋势与转录组测序结果相一致。结论:大麻二酚能够改善力竭运动引起的骨骼肌炎症反应。BACKGROUND:Cannabidiol is effective in ameliorating the body’s inflammatory response,but no clear mechanistic studies have been conducted to ameliorate skeletal muscle inflammation induced by exhaustive exercise.OBJECTIVE:To explore the mechanism by which cannabidiol improves skeletal muscle inflammation during exhaustive exercise by using transcriptome sequencing technology.METHODS:Thirty-six Sprague-Dawley rats were randomly divided into six groups:blank control group,exercise coconut oil group,exercise control group,50 mg/kg cannabidiol group,60 mg/kg cannabidiol group,and 70 mg/kg cannabidiol group,with six rats in each group.Except for rats in the blank control group,rats in each group were subjected to swimming exercise for 9 days to produce the exhaustive exercise model.At the end of each swimming exercise,rats in the cannabidiol groups were given 2 mL of fat-soluble cannabidiol at different concentrations(50,60,and 70 mg/kg)by gavage;rats in the exercise coconut oil group were given the same volume of coconut oil by gavage until the end of the exercise on the 9th day;and rats in the blank control group and the exercise control group were not given any special treatment.The levels of inflammatory factors and differentially expressed genes in the skeletal muscle of rats in each group were determined using ELISA and transcriptome sequencing techniques.Differentially expressed genes obtained were subjected to KEGG analysis,and the accuracy of the sequencing data was verified by fluorescence quantitative PCR.RESULTS AND CONCLUSION:The results of ELISA showed that the contents of interleukin-6(P<0.05),tumor necrosis factor-α(P<0.01),interleukin-10 and other inflammatory factors in the exercise group increased significantly compared with the blank control group and the coconut oil group.After cannabidiol intervention,the mass concentrations of interleukin-6 and tumor necrosis factor-αshowed a sequential decrease with increasing cannabidiol concentration.By comparing GO and KEGG databases,the functional prope

关 键 词：转录组 大麻二酚 力竭运动 骨骼肌炎症 差异基因 炎症因子 KEGG分析 炎症通路 

分 类 号：R496[医药卫生—康复医学] R318[医药卫生—临床医学] R875