油菜素内酯对盐碱胁迫下菊花幼苗生长及生理特性的影响  

Effects of brassinolide on growth and physiological characteristics of chrysanthemum seedlings under saline-alkali stress

作　　者：王玲玲[1] 虎淘淘 张昕怡 马潇源 张黎[1] 靳磊 严瑞 WANG Lingling;HU Taotao;ZHANG Xinyi;MA Xiaoyuan;ZHANG Li;JIN Lei;YAN Rui(College of Wine and Horticulture,Ningxia University,Yinchuan,Ningxia 750021,China;College of Forestry and Prataculture,Ningxia University,Yinchuan,Ningxia 750021,China)

机构地区：[1]宁夏大学葡萄酒与园艺学院,宁夏银川750021 [2]宁夏大学林业与草业学院,宁夏银川750021

出　　处：《湖南农业大学学报（自然科学版）》2024年第3期55-61,68,共8页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：2023年宁夏自然科学基金项目(2023AAC03147);2022年宁夏重点研发计划(2022BBF03035)。

摘　　要：以菊花耐盐品种‘绚秋江月’和盐敏感品种‘绚秋尽染’为试材,用NaCl和NaHCO3按1∶1(质量比)配制成100 mmol/L复合盐碱处理液,对园林小菊幼苗进行盐碱胁迫处理,对其叶片喷施不同浓度(0.001、0.010、0.100、1.000 mg/L)外源油菜素内酯(BR),以喷施H2O为对照(CK),研究BR对盐碱胁迫下园林小菊幼苗生长及生理指标的影响。结果表明:与对照相比,在盐碱胁迫下外源喷施0.100 mg/L BR,‘绚秋江月’和‘绚秋尽染’叶片相对含水量分别提高了8.11%、16.95%;SPAD值分别增加了9.55%和13.97%;相对电导率分别下降了27.63%和24.69%;丙二醛(MDA)含量分别下降了41.24%和67.38%;脯氨酸(Pro)含量分别提高了86.49%和69.46%;过氧化氢酶(CAT)活性分别增大了16.25%和18.14%;过氧化物酶(POD)活性分别增大了46.15%和68.38%;超氧化物歧化酶(SOD)活性分别增大了12.36%和16.32%;抗坏血酸过氧化物酶(APX)活性分别增大了76.88%和95.04%。外源喷施0.100 mg/L BR可以更好地促进盐碱胁迫下园林小菊幼苗的生长发育,提高抗氧化酶活性,增强生物膜的稳定性,从而起到缓解盐碱胁迫对菊花幼苗的伤害,且对盐敏感品种‘绚秋尽染’盐碱受害的缓解效果更佳。In this study,the salt-tolerant chrysanthemum cultivar‘Xuanqiu Jiangyue’and the salt-sensitive cultivar‘Xuanqiu Duran’were used as test materials,and NaCl and NaHCO3 were mixed with 1∶1 mass ration to prepare a compound saline-alkali solution at the concentration of 100 mmol/L.The seedlings of chrysanthemum were treated with saline-alkali stress,and the leaves were sprayed with four concentrations(0.001,0.010,0.100,1.000 mg/L)of exogenous brassinolide(BR)with the H2O as the control(CK).The effects of BR on the growth and physiological indexes of chrysanthemum seedlings under saline-alkali stress were studied.The results showed that compared with the control,the relative water contents of the leaves of‘Xuanqiu Jiangyue’and‘Xuanqiu Duran’increased by 8.11%and 16.95%,respectively,and the chlorophyll contents increased by 9.55%and 13.97%,respectively,when sprayed with 0.100 mg/L BR under saline-alkali stress.The relative conductivities decreased by 27.63%and 24.69%,respectively.The contents of malondialdehyde(MDA)decreased by 41.24%and 67.38%,respectively.The contents of proline(Pro)increased by 86.49%and 69.46%.The activities of catalase(CAT)increased by 16.25%and 18.14%,respectively.The activities of peroxidase(POD)increased by 46.15%and 68.38%,respectively.The activities of superoxide dismutase(SOD)increased by 12.36%and 16.32%,respectively.The activities of ascorbate peroxidase(APX)increased by 76.88%and 95.04%,respectively.In summary,exogenous spraying of 0.100 mg/L BR could better promote the growth and development of chrysanthemum seedlings under saline-alkali stress,improve the activity of antioxidant enzymes and the stability of biofilms,thereby alleviate the damage of saline-alkali stress to chrysanthemum seedlings,and the alleviation effect of salt-alkali damage of salt-sensitive variety‘Xuanqiu Duran’was more obvious.

关 键 词：园林小菊 外源油菜素内酯 盐碱胁迫 生理特性 

分 类 号：S682.11[农业科学—观赏园艺]