羊奶体细胞数与奶山羊瘤胃微生物区系的相关性  

The correlation between somatic cell count in goat milk and rumen microflora of dairy goats

作　　者：范斯琴 周宇桥 李虹佳 龚新宇 雍丛燕 屈金涛 简耀威 杨玲媛[1] FAN Siqin;ZHOU Yuqiao;LI Hongjia;GONG Xinyu;YONG Congyan;QU Jintao;JIAN Yaowei;YANG Lingyuan(College of Animal Science and Technology,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Shanmu Husbandry Co.Ltd,Chengbu,Hunan 422500,China)

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]湖南羴牧牧业有限公司,湖南城步422500

出　　处：《湖南农业大学学报（自然科学版）》2024年第3期76-82,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省草食动物产业技术体系营养与饲料岗位项目(湘农发【2019】105号);湖南省重大科技专项(2017 NK1020)。

摘　　要：选择120只(2.5±0.2)胎次、体质量(54.84±4.21)kg、泌乳日龄(72.0±3.5)d的健康西农萨能奶山羊,饲养42 d后收集奶样,测定羊奶中体细胞数(SCC),按照SCC将奶山羊分为3组,L–SCC组(SCC<5.0×10^(5)个/mL),M–SCC组(5.0×10^(5)个/mL≤SCC<1.0×10^(6)个/mL),H–SCC组(SCC≥1.0×10^(6)个/mL),从每组随机选取15只奶山羊,收集瘤胃液,测定瘤胃的微生物多样性,分析羊奶体细胞数与瘤胃微生物的相关性。结果表明:1)不同SCC水平奶山羊瘤胃微生物的α多样性、β多样性和优势菌群的差异不显著;L–SCC组放线菌门(Actinobacteriota)相对丰度显著高于M–SCC组和H–SCC组的,主要的差异菌属有解琥珀酸菌属(Succiniclasticum)、普氏菌UCG–003属(Prevotellaceae_UCG–003)、厌氧弧菌属(Anaerovibrio)、Gastranaerophilales_unclassified、双歧杆菌属(Bifidobacterium)、p–251–o5_unclassified、大肠杆菌–志贺菌属(Escherichia–Shigella)、夏普氏菌属(Sharpea)、乳杆菌属(Lactobacillus)、肠球菌属(Enterococcus);2)羊奶SCC与瘤胃解琥珀酸菌属的相对丰度呈显著正相关(P<0.05),与普氏菌UCG–003属的相对丰度呈极显著正相关(P<0.01)。120 healthy Xinong Saanen dairy goats with similar parity(2.5±0.2),body weight(54.84±4.21)kg,and lactation age(72.0±3.5)days were selected.After feeding 42 days,milk samples were collected and somatic cell counts(SCC)were determined.Based on SCC,the dairy goats were categorized into three groups:L-SCC group(SCC<5.0×10^(5)/mL),M-SCC group(5.0×10^(5)≤SCC<1.0×10^(6)/mL),and H-SCC group(SCC>1.0×10^(6)/mL).Fifteen goats were randomly selected from each group Rumen fluid was collected from these goats to assess rumen microbial diversity and analyze the correlation between somatic cell count in goat milk and rumen microflora.The results indicated that:1)there were no significant differences inα-diversity,β-diversity,and dominant bacterial groups in the rumen microbiota among dairy goats with different SCC levels.At the phylum level,the Actinobacteriota in the L-SCC group was significantly higher than that in the M-SCC and H-SCC groups.At the genus level,notable differences were observed in Succiniclasticum,Prevotellaceae_UCG-003,Anaerovibrio,Gastranaerophilales_unclassified,Bifidobacterium,p-251-o5_unclassified,Escherichia-Shigella,Sharpea,Lactobacillus,and Enterococcus;2)SCC showed a significant positive correlation with the relative abundance of Succiniclasticum in the rumen and a highly significant positive correlation with the relative abundance of Prevotellaceae_UCG-003 in goat milk.

关 键 词：奶山羊 羊奶体细胞数 瘤胃微生物区系 相关性 

分 类 号：S858.272.6[农业科学—临床兽医学]