中国南海豆荚软珊瑚Lobophytum sp.的化学成分及其抗菌活性研究  

Chemical composition and antibacterial activities of the soft coral Lobophytum sp.from the South China Sea

作　　者：孙曼曼 曾艳波 许含 姚励功 郭跃伟 苏明智 SUN Manman;ZENG Yanbo;XU Han;YAO Ligong;GUO Yuewei;SU Mingzhi(Ocean College of Hebei Agricultural University,Qinhuangdao 066000,China;Shandong Laboratory of Yantai Drug Discovery,Bohai Rim Advanced Research Institute for Drug Discovery,Yantai 264117,China;Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;School of Pharmcy,Yantai University,Yantai 264000,China)

机构地区：[1]河北农业大学海洋学院,河北秦皇岛066000 [2]烟台新药创制山东省实验室/中科环渤海(烟台)药物高等研究院,山东烟台264117 [3]海南省海洋生物资源功能性成分研究与利用重点实验室,热带生物技术研究所,中国热带农业科学院,海南海口571101 [4]烟台大学药学院,山东烟台264000

出　　处：《热带海洋学报》2024年第4期189-197,共9页Journal of Tropical Oceanography

基　　金：国家自然科学基金(81991521);国家重点研发计划(2022YFC2804100)。

摘　　要：对采集自中国南海的豆荚软珊瑚Lobophytum sp.的化学成分及生物活性进行研究。采用硅胶柱色谱、凝胶柱色谱、半制备高效液相色谱等分离手段进行分离纯化,通过对1H NMR、13C NMR等波谱数据分析并对比相关参考文献数据,确定了化合物的结构。从豆荚软珊瑚Lobophytum sp.中共分离鉴定了12个化合物,包括9个二萜类化合物:(+)-(7R,8R)-epoxy-7,8-dihydrocembrene C(1)、(1R,2E,4R,7E,11E)-cembra-2,7,11-triene-4-ol(2)、(-)-(2R,7R,8R)-sarcophytoxide(3)、entsarcophine(4)、sarcotenusene C(5)、(7S,8S)-epoxy-1,3,11-cembratrien-16-oic methyl ester(6)、sarcophytonin B(7)、sarcoconvolutum D(8)、laevigatol A(9);3个甾体类化合物:菜油甾醇(10)、(24S)-24-甲基胆甾醇(11)、柳珊瑚甾醇(12)。化合物8为首次从豆荚软珊瑚Lobophytum sp.中分离得到。化合物3、4、5、7、10对鱼类致病菌美人鱼发光杆菌FP2244、耐土霉素的副乳房链球菌SPOF3K,以及人类致病菌耐万古霉素屎肠球菌均具有显著的抗菌活性。To investigate the chemical composition and biological activity of the soft coral Lobophytum sp.collected from South China Sea.A series of methods such as silica gel column chromatography,thin-layer chromatography,Sephadex LH-20 column chromatography,and semi-preparative high performance liquid chromatography were used to separate and purify.The structure of the compounds was characterized by analysis of NMR data and comparison with the data reported in literature.Twelve compounds were identified from the soft coral Lobophytum sp.,including nine diterpenoids:(+)-(7R,8R)-epoxy-7,8-dihydrocembrene C(1),(1R,2E,4R,7E,11E)-cembra-2,7,11-triene-4-ol(2),(−)-(2R,7R,8R)-sarcophytoxide(3),entsarcophine(4),sarcotenusene C(5),(7S,8S)-epoxy-1,3,11-cembratrien-16-oic methyl ester(6),sarcophytonin B(7),sarcoconvolutum D(8),laevigatol A(9);three steroids:campesterol(10),(24S)-24-methylcholesterol(11),gorgonol(12).Compound 8 was isolated from the soft coral Lobophytum sp.for the first time.The bioassay results revealed that compounds 3,4,5,7 and 10 show significant antibacterial activity against fish pathogens Photobacterium damselae FP2244,oxytetracycline-resistant Streptococcus parauberis SPOF3K,and human pathogen vancomycin-resistant Enterococcus faecium.

关 键 词：软珊瑚 Lobophytum sp. 二萜 甾体 抗菌活性 耐药菌 

分 类 号：O629[理学—有机化学]