PD-1/PD-L1在MDS原始细胞、T细胞亚群和Treg细胞中的表达及意义  

Expression and Significance of PD-1/PD-L1 in MDS Blast Cells,T Lymphocyte Cell Subsets and Treg Cells

作　　者：王跃飞 陈葆国 郑瑞 俞依妮 WANG Yue-Fei;CHEN Bao-Guo;ZHENG Rui;YU Yi-Ni(Department of Pediatrics,Linhai Branch of the Second Affiliated Hospital of Zhejiang University(First People’s Hospital of Linhai City);The Central Laboratory,Taizhou Hospital Affiliated to Wenzhou Medical University,Linhai 317000,Zhejiang Province,China)

机构地区：[1]浙江大学附属二院临海分院(临海市第一人民医院)儿内科 [2]温州医科大学附属台州医院中心实验室,浙江临海317000

出　　处：《中国实验血液学杂志》2024年第4期1152-1159,共8页Journal of Experimental Hematology

基　　金：台州市科技局基金:(1801ky16)。

摘　　要：目的:探讨PD-1/PD-L1在MDS原始细胞、T细胞亚群和Treg细胞中的表达及其意义。方法:以88例MDS患者和19例AML患者为研究对象,以缺铁性贫血和健康骨髓捐献者为对照。采用流式细胞术分别检测其MDS/AML原始细胞、T细胞亚群和Treg细胞中PD-1/PD-L1的表达,同时检测外周血清Th1/Th2/Th17相关细胞因子的表达水平。结果:低危MDS组原始细胞、T细胞亚群、Treg细胞中PD-1/PD-L1的表达水平均明显低于对照组、中高危MDS组和AML组(均P<0.01);细胞因子以Th1/Th17类型占优势。中高危MDS组和AML组原始细胞、T细胞亚群、Treg细胞中的PD-1/PD-L1表达均明显高于对照组和低危MDS组(均P<0.01);细胞因子以Th2类型和Treg类型(IL-10、TGF-β)占优势。治疗后,MDS缓解组原始细胞、T细胞亚群、Treg细胞中PD-1/PD-L1的表达较对照组均无明显差异(P>0.05)。MDS未缓解组原始细胞、T细胞亚群、Treg细胞中PD-1/PD-L1的表达均显著高于MDS缓解组和对照组(P<0.01)。结论:PD-1/PD-L1在MDS患者中存在高表达,Treg(IL-10、TGF-β)和Th2相关细胞因子占优势以及效应T细胞被抑制的微环境,有利于肿瘤细胞增殖和免疫逃逸,可能促进了MDS疾病的进展。Objective:To investigate the expression and significance of PD-1/PD-L1 in MDS blast cells,T lymphocyte cell subsets and Treg cells.Methods:Eighty-eight MDS patients and 19 AML patients were collectd as the study subjects,and Iron deficiency anemia and healthy bone marrow donors were used as control group.The expression of PD-1/PD-L1 in MDS/AML blast cells,T lymphocyte cell subsets and Treg cells was detected by flow cytometry,and the expression level of Th1/Th2/Th17-related cytokines in peripheral serum was detected.Results:The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells in low risk MDS group were lower than that in control group,medium and high risk MDS group and AML group(all P<0.01),and Th1/Th17 type cytokines were dominant.The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells of intermediate and high risk MDS group and AML group were higher than that of control group and low risk MDS group(all P<0.01),and Th2 type and Treg type(IL-10、TGF-β)cytokines were dominant.After treatment,the differences of PD-1/PD-L1 expression were not statisticatly significant in blast cells,T lymphocyte cell subsets and Treg cells between the MDS remission group and the control group(all P>0.05).The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells in MDS non-remission group were significantly higher than that in remission group and control group(all P<0.01).Conclusion:The high expression of PD-1/PD-L1,dominance of Treg(IL-10、TGF-β)and Th2-related cytokines and inhibition of effector T lymphocyte cells in patients with MDS is conducive to tumor cell proliferation and immune escape,which may promote the progression of MDS disease.

关 键 词：骨髓增生异常综合征 PD-1/PD-L1 T细胞亚群 TREG细胞 

分 类 号：R551.3[医药卫生—血液循环系统疾病]