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作 者:敬强安 周超婷 吴韵怡 柯霞 童向民[1,2] JING Qiang-An;ZHOU Chao-Ting;WU Yun-Yi;KE Xia;TONG Xiang-Min(Institute of Clinical Medicine,Zhejiang Provincial People′s Hospital,People′s Hospital of Hangzhou Medical College,Hangzhou 310006,Zhejiang Province,China;College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,Zhejiang Province,China)
机构地区:[1]浙江省人民医院(杭州医学院附属人民医院)临床医学研究所,浙江杭州310006 [2]浙江工业大学生物工程学院,浙江杭州310014
出 处:《中国实验血液学杂志》2024年第4期1181-1185,共5页Journal of Experimental Hematology
摘 要:目的:研究6-异丙基双硫-2′-脱氧鸟苷(thiotert)对骨髓增生异常综合征(MDS)细胞SKM-1增殖及凋亡的影响。方法:采用CCK-8法检测thiotert对SKM-1细胞活力的抑制及GSH、NAC和Z-VAD-FMK对thiotert细胞毒性的逆转作用;Ed U细胞增殖检测试剂盒检测细胞增殖情况;流式细胞术检测细胞内的活性氧(ROS)水平;Western blot检测DNA损伤及凋亡相关蛋白的表达。结果:thiotert显著抑制了SKM-1细胞的增殖能力,诱导SKM-1细胞中ROS水平升高;thiotert处理导致SKM-1细胞内C-PARP、C-Caspase 3和γ-H2AX蛋白表达上调,而BCL-2显著下调;抗氧化剂GSH和NAC及凋亡抑制剂Z-VAD-FMK能部分逆转thiotert对细胞活力的抑制作用。结论:thiotert能够通过介导ROS的产生和促凋亡蛋白的表达来促进MDS细胞发生凋亡。Objective:To explore whether thiotert treatment can inhibit proliferation and induce apoptosis in myelodysplastic syndromes(MDS)cells.Methods:CCK-8 assay was used for determining the cytotoxicity of thiotert to MDS cell line SKM-1 and the reversal effect of GSH,NAC,and Z-VAD-FMK on thiotert-induced inhibition of cell viability.Ed U assay was deployed to detect the cell proliferation ability.Intracellular reactive oxygen species(ROS)was measured by flow cytometry after DCFH-DA staining.The expression of DNA damage-and apoptosis-related proteins was detected by Western blot.Results:Thiotert treatment significantly suppressed the cell viability and proliferation ability in SKM-1 cells.A large amount of ROS generation and markedly elevated C-PARP,C-Caspase 3,andγ-H2AX were observed after thiotert administration,while BCL-2 was significantly decreased.In addition,GSH,NAC,and Z-VAD-FMK were able to mitigate the cytotoxicity of thiotert on SKM-1 cells.Conclusion:Thiotert can promote MDS cell apoptosis by mediating ROS production and pro-apoptotic proteins expression.
关 键 词:骨髓增生异常综合征 6-异丙基双硫-2′-脱氧鸟苷 活性氧 凋亡
分 类 号:R551.3[医药卫生—血液循环系统疾病]
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