吸收放散法相对定量获取血浆IgG血型抗体的研究  

Relative Quantification for Obtaining the IgG Blood Group Antibodies in Plasma by the Absorption and Elution Test

作　　者：戚曦 高灵宝[2] 陈盛霞 QI Xi;GAO Lin-Bao;CHEN Sheng-Xia(Department of Laboratory Medicine,School of Medicine,Jiangsu University,Zhenjiang 212013,Jiangsu Prowince,China;Department of Tranisfiusion,The People's Hospital of Taizhou,Taizhou 225300,Jiangsu Province,China)

机构地区：[1]江苏大学医学院检验系,江苏镇江212013 [2]泰州市人民医院输血科,江苏泰州225300

出　　处：《中国实验血液学杂志》2024年第4期1278-1283,共6页Journal of Experimental Hematology

基　　金：科技部基础平台资助项目(2019-194-30)。

摘　　要：目的:建立可相对定量获取O型RhD+孕妇血浆IgG抗A、抗B血型抗体的吸收放散法。方法:随机选取95例O型RhD+孕妇血浆,用37℃温吸收、56℃热放散的方法获取血浆抗A、抗B血型抗体,以微柱凝胶抗人球蛋白试验分别测定放散液和血浆IgG抗A、抗B抗体效价,对比分析放散液与血浆抗体效价的差异及相关性。结果:放散液与血浆IgG抗A、抗B抗体效价分别作对数转换(Log2)后,放散液与血浆IgG抗A抗体效价差值与抗B抗体效价差值之间无统计学差异(P>0.05),放散液IgG抗体效价与血浆IgG抗体效价呈正相关(r=0.914),经散点图拟合直线方程为Y=-3.55+0.96X。结论:本吸收放散法可相对定量获取IgG抗体效价为8以上的O型RhD+孕妇血浆IgG抗A、抗B血型抗体,同时获取IgG抗A与抗B抗体的效果无差异,且获取的IgG血型抗体浓度能够反映血浆中相应抗体浓度水平。Objective:To establish the absorption and elution test for relatively quantitive obtaining anti-A and anti-B blood group IgG antibodies in the plasma of O-type RhD-positive pregnant women.Methods:95 cases of the O-type RhD-positive pregnant women plasma samples were randomly selected for obtaining the IgG antibodies of anti-A and anti-B blood group,with absorption test under 37℃and elution test under 56℃,and the IgG anti-A and anti-B antibody titers of plasma and elution were determined by the microcolumn gel anti-human globulin test.The differences and correlation between the titers of IgG antibodies in the eluent and plasma were compared and analyzed.Results:After a logarithmic transformation(Log2),there was no statistically difference between IgG antibody anti-A difference value and anti-B difference value in the eluent and plasma(P>0.05).The titer of IgG antibody in the eluent was positively correlated with the titer of IgG antibody in the plasma(r=0.914).The linear equation for IgG antibody titers fitted by a scatter plot between the eluent and plasma was Y=-3.55+0.96X.Conclusion:The absorption and elution test can be used to obtain the anti-A and anti-B IgG antibodies in the plasma of O-type RhD-positive pregnant women,whose plasma origin IgG titer is greater than 8.Meanwhile,the acquisition of anti-A antibodies was as effective as anti-B antibodies at the same time,and the antibodies obtained are positive proportional to their respective concentrations in the plasma.

关 键 词：吸收放散法 IGG血型抗体 母婴ABO血型不合 胎儿新生儿溶血病 

分 类 号：R446.6[医药卫生—诊断学]