菠菜HD-ZIP Ⅲ基因家族鉴定与表达分析  

Identification and expression analysis of HD-ZIPⅢgene family in spinach

作　　者：刘梓涵 张琪悦 袁满 翁莉 邵锋 顾立君 蔡晓锋[1] LIU Zihan;ZHANG Qiyue;YUAN Man;WENG Li;SHAO Feng;GU Lijun;CAI Xiaofeng(College of Life Sciences,Shanghai Normal University,Shanghai 200234,China;Shanghai Chongming Vegetable Science and Technology Extension Station,Shanghai 202150,China)

机构地区：[1]上海师范大学生命科学学院,上海200234 [2]上海市崇明区蔬菜科学技术推广站,上海202150

出　　处：《上海师范大学学报（自然科学版中英文）》2024年第3期352-361,共10页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海市科技创新行动计划农业科技领域项目(21N11900500)。

摘　　要：植物叶片在生长发育过程中,近-远轴极性的建立与维持是叶片正常行使光合效应、呼吸作用、蒸腾作用的结构基础.HD-ZIPⅢ是重要的叶片近轴极性基因,本研究在菠菜(Spinacia oleracea)基因组中鉴定到3个HD-ZIPⅢ基因家族成员,依次命名为SoHB1,SoHB2和SoHB3,并对其蛋白理化性质、保守结构域和进化关系进行了分析.研究结果表明:3个HD-ZIPⅢ蛋白都具有4个保守结构域.共线性分析表明:菠菜SoHB1,SoHB2和SoHB3分别与拟南芥ATHB8,ATREV和ATHB15存在共线性关系.启动子顺式作用元件预测结果表明:菠菜HD-ZIPⅢ家族基因启动子上游包含光响应、逆境胁迫反应、激素响应等顺式作用元件.在不同叶型菠菜种质中的表达模式分析结果表明:单戟型叶片的SoHBs表达量最高;此外,经干旱胁迫处理后,SoHB1,SoHB2和SoHB3的基因表达量普遍下降;对菠菜包括根、茎、薹叶、功能叶、薹叶柄、功能叶柄等部位进行表达分析,发现SoHB1在根部表达最为丰富,SoHB2和SoHB3在薹叶处表达最为丰富.During the growth and development of plant leaves,the establishment and maintenance of paraxial and abaxial polarity are essential for the normal functioning of photosynthesis,respiration and transpiration.HD-ZIPⅢgenes play a crucial role in establishing leaf adaxial polarity.In this study,three HD-ZIPⅢfamily genes were identified in the spinach genome,and designated as SoHB1,SoHB2 and SoHB3.Their physicochemical properties,conserved domains and evolutionary relationships were analyzed.The results showed that all three HD-ZIPⅢproteins possessed four conserved domains.Collinear analysis indicated that spinach SoHB1,SoHB2 and SoHB3 exhibited collinear relationships with Arabidopsis ATHB8,ATREV and ATHB15 respectively.The prediction results of promoter cis-acting elements reveled that the promoters of spinach HD-ZIPⅢfamily genes contained cis-acting elements related to light response,stress response and hormone response.Analysis of expression patterns in spinach germplasms with different leaf types indicated that the single halberd leaf exhibited the highest expression of HD-ZIPⅢ.Furthermore,the expression levels of SoHB1,SoHB2 and SoHB3 genes decreased following drought stress treatment.Expression analysis across various parts of spinach,including roots,stems,bolting leaves,functional leaves,bolting petioles,functional petioles and others,showed that SoHB1 was predominantly expressed in roots,while SoHB2 and SoHB3 were predominantly expressed in bolting leaves.

关 键 词：菠菜(Spinacia oleracea) HD-ZIPⅢ基因家族 干旱胁迫 基因表达量 

分 类 号：S636.1[农业科学—蔬菜学]