紫丁香苷联合吉西他滨对胰腺癌细胞生长的影响  

Effect of syringin combined with gemcitabine on the growth of pancreatic cancer cells

作　　者：侯颖征 陈静 Hou Yingzheng;Chen Jing(College of Life Sciences,North China University of Science and Technology,Tangshan 063210,China)

机构地区：[1]华北理工大学生命科学学院,河北唐山063210

出　　处：《华北理工大学学报（医学版）》2024年第4期260-266,共7页Journal of North China University of Science and Technology：Health Sciences Edition

基　　金：河北省自然科学基金项目(编号:H2021209004)。

摘　　要：目的探究紫丁香苷联合吉西他滨对胰腺癌细胞生长的影响,并预测其可能的分子机制。方法(1)应用GDSC数据库,从三种不同类型的胰腺癌细胞中,筛选出对吉西他滨耐药性最强的细胞。(2)MTT方法检测紫丁香苷、吉西他滨以及两者联用对人胰腺癌细胞AsPC-1存活率的影响,并得出紫丁香苷、吉西他滨作用于AsPC-1细胞的半数抑制浓度(IC50)。(3)台盼蓝实验进一步验证联合用药对AsPC-1细胞的影响。(4)通过CTD数据库寻找与紫丁香苷和吉西他滨有关的基因,并绘制韦恩图,得出共同基因。(5)使用Kaplan-Meier plotter数据库对筛选出的共同基因进行生存分析,得到与紫丁香苷和吉西他滨联合用药的作用密切相关的基因。结果(1)三种胰腺癌细胞中耐药性最强的细胞为AsPC-1细胞。(2)吉西他滨、紫丁香苷以及两者联用均可抑制AsPC-1细胞的存活率:紫丁香苷的IC50值为(41.76±0.37)mmol/L,吉西他滨的IC50值为(0.01±0.07)mmol/L。(3)与紫丁香苷和吉西他滨有关的基因包括TNF、MPO、NFKBIA、IFNG、IL-4、IL-13、RELA和IL-6。(4)与联合用药作用有关的基因包括TNF、IL-13和IFNG。结论紫丁香苷与吉西他滨联合应用可能通过TNF、IL-13和IFNG基因抑制胰腺癌细胞的生长。Objective To investigate the effect of syringin combined with gemcitabine on the growth of pancreatic cancer cells and predict its possible molecular mechanism.Methods(1)Using the GDSC database,the pancreatic cancer cell with the strongest resistance to gemcitabine was screened out from three different types of pancreatic cancer cells.(2)MTT assay was used to detect the effects of syringin,gemcitabine,and their combination on the survival rate of human pancreatic cancer cell line AsPC-1,and the half-maximal inhibitory concentration(IC 50)of syringin,gemcitabine,on AsPC-1 cells was obtained.(3)Trypan blue assay was further used to verify the effect of combined drug use on AsPC-1 cells.(4)Genes related to syringin and gemcitabine were searched through the CTD database,and a Venn diagram was drawn to identify common genes.(5)The Kaplan-Meierplotter database was used to perform survival analysis on the selected common genes to identify genes closely related to the combined use of syringin and gemcitabine.Results(1)Among the three pancreatic cancer cell lines,AsPC-1 cells exhibited the strongest resistance to gemcitabine.(2)Syringin,gemcitabine,and their combination all inhibited the survival rate of AsPC-1 cells:the IC 50 value of syringin was(41.76±0.37)mmol/L,the IC 50 value of gemcitabine was(0.01±0.07)mmol/L.(3)Genes related to syringin and gemcitabine included TNF,MPO,NFKBIA,IFNG,IL-4,IL-13,RELA,and IL-6.(4)Genes related to the effect of combined drug use included TNF,IL-13,and IFNG.Conclusion The combined application of syringin and gemcitabine may inhibit the growth of pancreatic cancer cells through TNF,IL-13,and IFNG genes.

关 键 词：紫丁香苷 吉西他滨 胰腺癌 联合用药 

分 类 号：R966[医药卫生—药理学]