头孢菌素菌渣有机肥对玉米土壤中耐药菌及相关抗性基因的影响  

Effect of cephalosporin residue organic fertilizer on resistant bacteria and related resistance genes in maize soil

作　　者：刘婉琴 易鸳鸯 彭小武 谢芳 顾美英[2] 张志东 LIU Wanqin;YI Yuanyang;PENG Xiaowu;XIE Fang;GU Meiying;ZHANG Zhidong(College of Life Science and Technology,Xinjiang University,Urumqi 830047,China;Xinjiang Special Environment Microbiology Laboratory/Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Xinjiang Academy of Environmental Protection Sciences,Urumqi 830011,China;Xinjiang Cleaner Production Engineering Technology Research Center,Urumqi 830011,China;National Environmental Protection Research Station of Junggar Desert-Oasis Interleaving Area,Urumqi 830011,China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830047 [2]新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐830091 [3]新疆环境保护科学研究院,乌鲁木齐830011 [4]新疆清洁生产工程技术研究中心,乌鲁木齐830011 [5]国家环境保护准噶尔荒漠绿洲交错区科学观测研究站,乌鲁木齐830011

出　　处：《新疆农业科学》2024年第4期1003-1010,共8页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区青年科技拔尖人才项目-青年科技创新人才培养(2022TSYCCX0067);新疆农业科学院科技创新重点培育专项(xjkcpy-2021002);新疆农业科学院自主培育项目团队建设专项(nkyzztd-001);新疆农业科学院农业科技创新平台能力提升建设专项(nkypt005)。

摘　　要：【目的】研究头孢菌素菌渣有机肥施用后对玉米农田土壤中耐药菌及相关抗性基因丰度的影响,为头孢菌素菌渣有机肥施用生物安全性评价提供科学依据。【方法】设置不施菌渣有机肥(CK)、施用菌渣有机肥500 kg/667m^(2)(B_(1))、1000 kg/667m^(2)(B_(2))3个处理,分别采用可培养方法和实时荧光定量检测,分析各处理下玉米农田中耐药菌及相关抗性基因丰度变化。【结果】施用头孢菌素菌渣有机肥能显著提高玉米苗期和结果期土壤中细菌总数及苗期时头孢拉定耐药菌菌数。获得了主要抗生素耐药菌株41株,其中,头孢拉定耐药菌8株,分属于6个属。【结论】施用头孢菌素菌渣有机肥能显著提高玉米苗期土壤中各ARGs基因的相对丰度,但在结果期各处理间β-内酰胺类ARGs基因bla TEM相对丰度无显著影响,并对其它耐药基因无明显规律性影响。【Objective】To explore the effect of cephalosporin residue organic fertilizer on the abundance of drug-resistant bacteria and related resistance genes in the maize soil,and to provide scientific basis for biological safety evaluation in the application of cephalosporin residue organic fertilizer.【Methods】Three experimental groups were set up,which included none organic fertilize treatment(CK),the organic fertilizer treatments with 500 kg/667 m^(2)(B_(1))and with 1000 kg/667 m^(2)(B_(2)),respectively.The antibiotic resistant bacteria and related antibiotic resistance genes(ARGs)with different treatments were analyzed by using the cultivable method and the real-time fluorescence quantitative PCR.【Results】The results showed that the total number of bacteria was significantly increased in the treatments with cephalosporin residue organic fertilizer,and the number of cefradine resistant bacteria was significantly increased in the treatments at corn seedling stage as well.Forty-one strains of antibiotic-resistant strains were obtained,among which 8 cefradine resistant strains belonged to 6 genera.【Conclusion】The relative abundance of ARGs genes in corn seedling soil was significantly increased in the treatments with cephalosporin residue organic fertilizer.There were no significant effects on the relative abundance of bla TEM gene in the treatments at the fruiting stage,and none regular influence on other drug-resistant genes.

关 键 词：头孢菌素菌渣 玉米土壤 耐药菌 ARGs Q-PCR 

分 类 号：S512[农业科学—作物学]