中国不同城市家用洗碗机中微生物多样性分析  

Analysis of Microbial Diversity in Household Dishwashers in China’s Different Cities

作　　者：许华 张晶晶 白飞荣 张天赐 高逸 张健 葛媛媛 蔡延涛 杨子睿 杨春龙 康佳丽 姚粟 XU Hua;ZHANG Jing-jing;BAI Fei-rong;ZHANG Tian-ci;GAO Yi;ZHANG Jian;GE Yuan-yuan;CAI Yan-tao;YANG Zi-rui;YANG Chun-long;KANG Jia-li;YAO Su(Foshan Shun Midea Washing Applience Manufacturing Co.,Ltd.;China National Research Institute of Food&Fermentation Industries Co.,Ltd.;China Standard Certification Beijing Co.,Ltd.)

机构地区：[1]佛山市顺德区美的洗涤电器制造有限公司 [2]中国食品发酵工业研究院有限公司 [3]中标能效科技(北京)有限公司

出　　处：《中国标准化》2024年第13期273-282,共10页China Standardization

摘　　要：本文采用平板培养法和高通量测序技术全面解析冬季中国不同城市家用洗碗机微生物的群落结构。结果表明,基于可培养技术共分离获得245株微生物菌种,涵盖56个属。优势菌属主要有芽孢杆菌属(Bacillus)、埃希氏杆菌属(Escherichia)、纤维芽孢杆菌属(Cytobacillus)、曲霉属(Aspergillus)、青霉属(Penicillium)、假丝酵母属(Candida)等。通过高通量测序,发现洗碗机中物种多样性和群落丰富度较高,不同城市洗碗机样品微生物群落结构有一定差异,本研究为定向筛选洗碗机中优势微生物资源奠定基础,为洗碗机抗菌、抑菌评价试验的标准菌株开发提供了数据支撑。In this paper,plate culture method and high-throughput sequencing technology were used to analyze the microbial community structure of household dishwashers in different cities in China in winter.In result,a total of 245 strains,covering 56 genera,were isolated based on culturable techniques.The dominant bacteria genera are mainly Bacillus,Escherichia,Cytobacillus,Aspergillus,Penicillium,and Candida.Through high-throughput sequencing,it was found that the species diversity and community richness of dishwashers were high,and the microbial community structure of dishwashers samples in different cities was different to some extent.This study laid a foundation for the targeted screening of dominant microbial resources in dishwashers,and provided data support for the development of standard strains for antibacterial and bacteriological evaluation tests of dishwashers.

关 键 词：洗碗机 微生物 高通量测序技术 多样性 

分 类 号：TM925.58[电气工程—电力电子与电力传动]