黄颡鱼XX伪雄鱼制备与全雌种群规模化繁育  

INDUCTION OF XX GENOTYPIC PSEUDO-MALES AND LARGE-SCALE REPRODUCTION OF ALL-FEMALE POPULATION IN YELLOW CATFISH(TACHYSURUS FULVIDRACO)

作　　者：丁洋 鲁子怡 刘娅 曹霄 王亚 梁浩文 任旌雷 邓翔 何焱[1] 樊启学 牟长军 沈志刚[1] DING Yang;LU Zi-Yi;LIU Ya;CAO Xiao;ANG Ya;LIANG Hao-Wen;REN Jing-Lei;ENG Xiang;HE Yan;FAN Qi-Xue;MU Chang-Jun;SHEN Zhi-Gang(Key Laboratory of Freshwater Animal Breeding,Ministry of Agriculture,Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt,Ministry of Education,Hubei Provincial Engineering Laboratory for Pond Aquaculture,College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Hubei Huangyouyuan Fishery Development Limited Company,Wuhan 430070,China;Fisheries Development Service Center of Weishan County,Jining 277699,China)

机构地区：[1]华中农业大学水产学院,农业农村部淡水生物繁育重点实验室,教育部长江经济带大宗水生生物产业绿色发展工程研究中心,池塘健康养殖湖北省工程实验室,武汉430070 [2]湖北黄优源渔业发展有限公司,武汉430299 [3]微山县渔业发展服务中心,济宁277699

出　　处：《水生生物学报》2024年第8期1365-1377,共13页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金面上项目(32273122);科技创新2030-重大项目(2023ZD04068)资助。

摘　　要：研究以XX遗传型全雌黄颡鱼(Tachysurus fulvidraco)群体为实验材料,利用加氢甲基睾酮(17α-methyldihydrotestosterone,MDHT)和来曲唑(Letrozole,LZ)对性别分化关键时期(12-60日龄)黄颡鱼幼鱼进行投喂处理,设置MH组(50 mg/kg MDHT)、ML组(10 mg/kg MDHT)、MH+L组(50 mg/kg MDHT,1000 mg/kg LZ)、ML+L组(10 mg/kg MDHT,1000 mg/kg LZ)及对照CR组(0 MDHT,0 LZ)。结果显示,ML组和ML+L组雄性化个体比例较高,分别为66.7%和83.3%,其性腺类型包括间性性腺(37.5%和50.0%)和全精巢性腺(29.2%和33.3%),且两组中均获得了43.8%的功能性XX伪雄鱼。以XX伪雄鱼为父本、以经多代选育的全雌黄颡鱼为母本,进行规模化人工繁殖,获得了280万尾XX遗传型黄颡鱼苗种。在92日龄时发现,在室外池塘养殖条件下,98.0%的个体卵巢发育良好,2.0%的个体性逆转为非功能性XX伪雄鱼。研究建立了一套黄颡鱼全雌种群规模化繁育技术体系,为黄颡鱼规模化人工繁育与遗传改良、黄颡鱼产业健康可持续发展提供保障。There is a critical shortage of female broodstock in the yellow catfish industry,making the production of allfemale populations using sex-control technology extremely important to meet the demand for seedling production and genetic improvement.In this study,XX genotypic yellow catfish fry were treated with 17a-methyldihydrotestosterone(MDHT)and Letrozole(LZ)through feed during the critical period of sex differentiation(12 to 60 days post-hatching,DPH).Five experimental groups were established:MH(50 mg/kg MDHT),ML(10 mg/kg MDHT),MH+L(50 mg/kg MDHT,1000 mg/kg LZ),ML+L(10 mg/kg MDHT,1000 mg/kg LZ),and control group(CR,0 MDHT,0 LZ).The ML and ML+L groups obtained the highest proportions of masculinization,at 66.7%and 83.3%,respectively.Sexreversed individuals in these groups included complete-testis-type(29.2%,33.3%)and intersex gonads(37.5%,50.0%),both of which produced 43.8%functional testes.Using XX pseudo-males as fathers and selecting XX females as mothers for multiple generations,2800000 XX genotypic fry were obtained through induced spawning.In an outdoor earthen pond culture environment,it was found that the ovaries of 98.0%of individuals developed well,while 2.0%of individuals reversed to phenotypic males at 92 DPH,At this point,our research team has established a large-scale technological system for producing all-female yellow catfish populations,providing crucial support for large-scale induced spawning and genetic improvement,and serving as an important guarantee for the healthy development of the yellow catfish industry.

关 键 词：性别控制 加氢甲基睾酮 来曲唑 全雌种群 雄性化 黄颡鱼 

分 类 号：S965.1[农业科学—水产养殖]