基于RNA-seq的大麦苗期抗旱相关基因的挖掘与分析  

RNA-seq-based mining and analysis of drought-related genes in barley seedlings

作　　者：鞠乐[1,2] 齐军仓 牛银亭[2] 石培春 宋瑞娇[1] 宋凌宇 阴志刚 陈培育 强学兰 JU Le;QI Juncang;NIU Yinting;SHI Peichun;SONG Ruijiao;SONG Lingyu;YIN Zhigang;CHEN Peiyu;QIANG Xuelan(College of Agriculture,Shihezi University,Shihezi Xinjiang 832003,China;Academy of Agricultural Sciences,Nanyang City,Nanyan Henan 473000,China;Bureau of Agriculture and Rural Development,Wancheng District,Nanyang Henan 473000,China)

机构地区：[1]石河子大学农学院,新疆石河子832003 [2]南阳市农业科学院,河南南阳473000 [3]宛城区农业农村局,河南南阳473000

出　　处：《新疆农业科学》2024年第5期1077-1084,共8页Xinjiang Agricultural Sciences

基　　金：财政部和农业农村部:国家现代农业产业技术体系项目(CARS-05-19B);国家自然科学基金项目(32360456)。

摘　　要：【目的】挖掘与分析大麦苗期抗旱相关基因,为研究大麦分子抗旱机制和选育抗旱大麦品种提供理论支撑。【方法】以啤酒大麦品种新啤6号为材料,应用转录组测序RNA-seq技术对干旱胁迫前后大麦苗期倒二叶叶片进行转录组测序,并采用实时荧光定量RT-PCR方法进行功能基因验证。【结果】(1)干旱胁迫前后新啤6号倒二叶中3835个差异表达基因,主要为编码ABC转运蛋白、核糖体蛋白、转录因子、脱水素、过氧化物酶、蛋白质磷酸酶等的基因。(2)DEG主要富集在淀粉和蔗糖代谢、转运蛋白、植物激素信号转导、伴侣和折叠催化剂、氨基糖和核苷酸糖的代谢、苯丙氨酸代谢、牛磺酸和次牛磺酸代谢、过氧化物酶体等途径。【结论】大麦干旱胁迫前后基因表达差异显著(其中上调基因1592个,下调基因2243个)。【Objective】To excavate the genes related to drought resistance in the hope of providing theoretical support for analyzing the molecular drought resistance mechanism of barley and guiding the drought resistance breeding of barley.【Methods】Taking New beer No.6 as the test material,we applied transcriptome sequencing RNA-seq technology to sequence the inverted bilobed leaves of barley seedlings before and after drought stress,and real-time fluorescence quantitative RT-PCR was used to verify the functional genes.【Results】(1)In this study,3835 differentially expressed genes were detected in the inverted bilobed of New beer 6 before and after drought stress by using RNA-seq,mainly genes encoded by ABC transporter proteins,ribosomal proteins,transcription factors,dehydrins,peroxidases,protein phosphatases,etc.The results showed that DEG was mainly enriched in starch and sucrose metabolism,transporter proteins,plant hormone signaling transduction,and protein phosphatases.(2)By KEGG metabolic pathway enrichment analysis,DEGs were mainly enriched in starch and sucrose metabolism,transporter proteins,phytohormone signaling,chaperones and folding catalysts,aminosugar and ribosugar metabolism,phenylalanine metabolism,taurine and hyposulphite metabolism,peroxisomes and other pathways.【Conclusion】Significant differences are shown in gene expression before and after drought stress in barley.The results of this study have laid the foundation for the excavation of key drought-resistant genes and for further analysis of drought-resistant mechanisms in barley(1592 up-regulated genes and 2243 down-regulated genes).

关 键 词：大麦 干旱胁迫 转录组 基因挖掘 

分 类 号：S512.3[农业科学—作物学]