PRRSV HNHK3-2021毒株的ORF5和ORF7序列分析  

Sequence analysis of ORF3 and ORF5 of PRRSV HNHK3-2021 strain isolated in Hainan

作　　者：范悦轩 马佳镁 黄春媛 郑佳馨 陈素贞 张艳[2] 刘光亮 曹宗喜 FAN Yuexuan;MA Jiamei;HUANG Chunyuan;ZHENG Jiaxin;CHEN Suzhen;ZHANG Yan;LIU Guangliang;CAO Zongxi(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;Hainan Provincial Key Laboratory of Tropical Animal Breeding and Disease Research,Institute of Animal Husbandry and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China;Hainan Provincial Academician Innovation Center,Institute of Animal Husbandry and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830000 [2]海南省农业科学院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室,海口571100 [3]海南省农业科学院畜牧兽医研究所海南省院士团队创新中心,海口571100

出　　处：《新疆农业科学》2024年第5期1292-1300,共9页Xinjiang Agricultural Sciences

基　　金：海南省省属科研院所技术创新专项(jscx202001);国家自然科学基金项目(32060796);家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2020KFKT021);海南省农业科学院院本级科研项目(HAAS2022PT0205);国家重点研发计划(2023YFC3404302)。

摘　　要：【目的】研究海南猪繁殖与呼吸综合征病毒PRRSV的流行病学特征和遗传变异规律。【方法】采集海南省海口市及周边地区样品,分离出PRRSV HNHK3-2021毒株,并测定NSP2基因以及ORF5和ORF7基因序列,采用Lasergene 7.1进行序列比对分析,用MEGA-X进行遗传演化分析。【结果】分离出的PRRSV HNHK3-2021株为美洲型毒株,PRRSV HNHK3-2021株和EF112447 HEB1、EU109503 GD、EU624117 XH-GD的ORF5和ORF7的核苷酸相似性较高,分别为99.2%~99.3%和99.5%~100%,推导氨基酸相似性为99%~99.5%和100%;PRRSV HNHK3-2021株和EU864232 SHB、AY262352_HB-2(sh)2002、AY656990 Abst-1、AY032626 CH-1a、EU807840 CH-1R的ORF5和ORF7的核苷酸相似性为87.1%~96.4%和91.4%~97.3%,推导氨基酸的相似性为86.6%~93%和91.9%~97.6%;PRRSV HNHK3-2021株和JN654459 NADC30、KP860909 FJZ03、MH068878 SD17-38、AY881994 FJ-1的ORF5和ORF7的核苷酸相似性为85.4%~87.1%和89%~91.4%。氨基酸的相似性为86.1%~87.1%和89.5%~91.9%。【结论】RRSV HNHK3-2021毒株和EU624117 XH-GD具有最近的遗传距离。【Objective】To explore the epidemiological characteristics and genetic variation of PRRSV in Hainan.【Methods】Samples were collected from Haikou and surrounding areas.The PRRSV strain HNHK3-2021 was isolated,and the NSP2 genes,as well as the ORF5 and ORF7 gene sequences,were determined.Sequence alignment analysis was conducted using Lasergene 7.1,and genetic evolution analysis was performed using MEGA-X.【Results】The PRRSV HNHK3-2021 strain isolated was found to be of American origin with high nucleotide similarity to EF112447 HEB1,EU109503 GD,and EU624117 XH-GD ORF5 and ORF7(99.2%-99.3%and 99.5%-100%,respectively).The derived amino acid similarity was 99%-99.5%and 100%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and EU864232 SHB,AY262352_HB-2(sh)2002,AY656990 Abst-1,AY032626 CH-1a,EU807840 CH-1R were found to be 87.1%-96.4%and 91.4%-97.3%,respectively.Similarly,the similarity of amino acids was 86.6%-93%and 91.9%-97.6%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and JN654459 NADC30,KP860909 FJZ03,MH068878 SD17-38,AY881994 FJ-1 were found to be 85.4%-87.1%and 89%-91.4%.The similarity of amino acids was 86.1%-87.1%and 89.5%-91.9%.【Conclusion】The PRRSV HNHK3-2021 strain and EU624117 XH-GD have the closest genetic distance.

关 键 词：猪繁殖与呼吸综合征 遗传进化 ORF5 ORF7 

分 类 号：S85[农业科学—兽医学]