Klebsiella oxytoca EN-B2高效转化羟胺和亚硝态氮的影响因素研究  

Study on the Characteristics of Efficient Conversion of Hydroxylamine and Nitrite by Klebsiella Oxytoca EN-B2

作　　者：邹建国 徐兴舟 王英 田珊 王小强 王嘉臣[1,2] 陈婵 邹婷 何腾霞 江龙 ZOU Jianguo;XU Xingzhou;WANG Ying;TIAN Shan;WANG Xiaoqiang;WANG Jiachen;CHEN Chan;ZOU Ting;HE Tengxia;JIANG Long(College of Life Sciences,Guizhou University,Guiyang 550025,China;Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学山地植物资源保护与种质创新教育部重点实验室,贵州贵阳550025

出　　处：《贵州大学学报（自然科学版）》2024年第4期116-124,共9页Journal of Guizhou University:Natural Sciences

基　　金：国家自然科学基金青年科学基金资助项目(42007223);贵州大学实验室开放项目(SYSKF2024-72);贵州大学科研创新团队资助项目(贵大科创团[2024]06号);大学生创新创业训练计划资助项目(贵大[省]创字[2023]110号)。

摘　　要：羟胺和亚硝态氮对动植物具有毒害作用,并通过污染环境进一步危害人类健康。本研究以实验室前期分离获得的一株高效转化羟胺和亚硝态氮的克雷伯氏菌(Klebsiella oxytoca)为研究对象,探究不同环境因素对该菌株同时去除羟胺和亚硝态氮的影响。结果表明,在碳源为丁二酸钠,接菌量0.4×10^(8) CFU、碳氮比(C/N)15、温度25℃、转速150 r/min和初始pH值8.0条件下,菌株EN-B2对羟胺、亚硝态氮、总氮的去除效率最高,去除率分别达到了99.42%、100.00%和87.76%。此外,本研究通过向亚硝态氮体系中添加不同浓度的羟胺,进一步探讨了羟胺对菌株EN-B2反硝化过程的影响。结果表明,当体系中羟胺浓度超过3 mg/L后,随着羟胺浓度的进一步增加,该菌株在相同时间下亚硝态氮去除速率降低。本研究有助于理解Klebsiella oxytoca EN-B2在羟胺和亚硝态氮混合体系中的除氮特性,为氮污染废水的处理工艺提供重要参考。Hydroxylamine and nitrite are toxic to plants and animals,and can further endanger human health through polluted environment.In this study,a strain of Klebsiella oxytoca EN-B2 with efficiently ability to convert hydroxylamine and nitrite was used,which isolated in our previous study.Exploring the effects of environmental factors on the removal of hydroxylamine and nitrite.The results show that EN-B2 remov hydroxylamine,nitrite and total nitrogen most efficiently under the conditions of sodium succinate as carbon source,0.4×10^(8) CFU/mL,carbon-nitrogen ratio(C/N)of 15,temperature of 25℃,rotation speed of 150 r/min and initial pH of 8.0.It with removal rates of 99.42%,100.00% and 87.76%,respectively.In addition,different concentrations of hydroxylamine were added to the nitrite system,and the effect of hydroxylamine on nitrite removal by strain EN-B2 was further explored.The results show when the concentration of hydroxylamine exceed 3 mg/L,as the concentration of hydroxylamine increase,the removal ability of EN-B2 decrease.This study is helpful for understanding the characteristics of Klebsiella Oxytoca EN-B2 in removing hydroxylamine and nitrite,which providing an important reference for the treatments of nitrogen contaminated wastewater.

关 键 词：Klebsiella oxytoca 羟胺 亚硝态氮 环境因素 

分 类 号：X523[环境科学与工程—环境工程]