基于光学检测的分子间相互作用表征技术研究进展  

Optical detection-based characterization techniques of molecular interactions:research progress

作　　者：封加栋 陆峰[1] FENG Jiadong;LU Feng(Department of Pharmaceutical Analysis,School of Pharmacy,Naval Medical University(Second Military Medical University),Shanghai 200433,China)

机构地区：[1]海军军医大学(第二军医大学)药学系药物分析学教研室,上海200433

出　　处：《海军军医大学学报》2024年第7期872-879,共8页Academic Journal of Naval Medical University

基　　金：国家自然科学基金面上项目(82273894).

摘　　要：分子间相互作用的表征技术是阐明细胞生物学事件、了解疾病发生机制、辅助药物研发的有力手段。近年来,分子间相互作用的表征技术发展迅速,不断向着高灵敏度、高通量、极短时间、极低检测限的方向发展。本文对表面等离子体共振、生物膜干涉、背向散射干涉以及微量热泳动这4种常见的、基于光学检测的分子间相互作用表征技术的原理、特点及最新应用进展进行了综述与比较,为分子间相互作用表征技术的选择提供参考。Characterization techniques of molecular interactions are powerful methods to elucidate cell biological events,understand the pathogenesis of diseases,and assist drug development.In recent years,the characterization techniques of molecular interactions have been developing rapidly and have been constantly moving toward high sensitivity,high throughput,extremely short time,and extremely low detection limits.This review summarizes the principles,characteristics,and latest application progress of 4 common optical detection-based characterization techniques of molecular interactions,including surface plasmon resonance,biolayer interferometry,back-scattering interferometry,and microscale thermophoresis,so as to provide a reference for the selection of characterization techniques of molecular interactions.

关 键 词：分子间相互作用 表面等离子体共振 生物膜干涉 背向散射干涉 微量热泳动 

分 类 号：O657.3[理学—分析化学] Q503[理学—化学]