2017-2020年华东地区猪源多杀性巴氏杆菌耐药性和PFGE分析  

Drug Resistance and PFGE Analysis of Pasteurella multocida Isolates of Pig Origin in East China from 2017 to 2020

作　　者：宋强强 轩慧勇 朱维 魏建超[2] 刘珂[2] 邵东华[2] 邱亚峰[2] 马志永[2] 李蓓蓓[2] 夏利宁[1] SONG Qiangqiang;XUAN Huiyong;ZHU Wei;WEI Jianchao;LIU Ke;SHAO Donghua;QIU Yafeng;MA Zhiyong;LI Beibei;XIA Lining(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China;Shanghai Veterinary Research Institute,CAAS,200241,China;Tengzhou Animal Disease Prevention and Control Center of Shandong Province,Tengzhou 277599,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]中国农业科学院上海兽医研究所,上海200241 [3]山东省滕州市动物疫病预防中心,滕州277599

出　　处：《中国动物传染病学报》2024年第3期128-133,共6页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金(31860714);上海市科技兴农技术创新项目(沪农科创字(2022)第2022-02-08-00-12-F01100号)。

摘　　要：为了解华东地区猪源多杀性巴氏杆菌的耐药情况及流行特征,本研究收集了2017—2020年华东地区分离的31株猪源多杀性巴氏杆菌,通过琼脂稀释法检测其对12种临床常用抗菌药物的最小抑菌浓度;采用脉冲场凝胶电泳(PFGE)方法进行分子分型探求菌株亲缘关系。结果显示:31株猪源多杀性巴氏杆菌,对复方新诺明的耐药率最高(61.29%),其次为泰妙菌素(32.26%)、氟苯尼考(25.81%)、多西环素(12.90%)和大观霉素(3.23%)。所有菌株对头孢噻呋、庆大霉素、阿莫西林、阿莫西林/克拉维酸、恩诺沙星完全敏感;耐药谱型呈现多样化特点,31株猪源多杀性巴氏杆菌共表现为8种耐药谱型,其中有2株3药耐药菌株,耐药谱型分别为DOX+TIA+SPT和FFC+TIA+SXT;依据85%相似性标准可将其分为14个PFGE分型,其中A、G、H、J簇共有19株菌,占61.29%,并且PFGE分型相同的菌株耐药表型也高度相似。研究结果表明,华东地区猪源多杀性巴氏杆菌呈现耐药模式和PFGE分子分型多样化的特性,PFGE分型与耐药表型之间存在一定的相关性,应加强猪源多杀性巴氏杆菌的耐药性监测和流行性防控。In order to investigate the drug resistance and epidemic characteristics of 31 Pasteurella multocida strains isolated from pigs in East China,minimum inhibitory concentrations(MICs)against 12 commonly used antibiotics were determined by agar dilution method and molecular typing was performed by pulsed field gel electrophoresis(PFGE).Highest resistance rate(61.29%)was observed for sulfamethoxazole,followed by tiamulin(32.26%),florfenicol(25.81%),doxycycline(12.90%)and spectinomycin(3.23%).All of these 31 strains were susceptible to ceftiofur,gentamicin,amoxicillin-clavulanic acid and enrofloxacin.These 31 P.multocida strains showed 8 drug resistance patterns,among which 2 strains exhibited resistance to 3 antibiotics(DOX+TIA+SPT and FFC+TIA+SXT).According to 85%similarity criteria,these 31 P.multocida strains were divided into 14 PFGE types,of which 19 strains(61.29%)clustered in four types,namely A,G,H and J.The antimicrobial resistance profiles of those strains with the same PFGE types were also highly similar.Our results demonstrated that P.multocida strains from pigs in East China showed diverse AMR patterns and PFGE types.There was a certain correlation between PFGE typing and antimicrobial resistance profiles.Monitoring of drug resistance and epidemic prevention and control of P.multocida from pigs should be strengthened in the future work.

关 键 词：多杀性巴氏杆菌 耐药性 PFGE 猪 

分 类 号：S852.61[农业科学—基础兽医学]