2020年国内部分地区奶牛乳腺炎源大肠杆菌的分子流行病学调查  

Molecular Epidemiological Investigation of Escherichia Coli from Cow Mastitis in 2020 in Some Areas of China

作　　者：买尔哈巴·吾斯曼 左佳坤 吕朝阳 蒋蔚[2] 陈兆国[2] 张秀萍[1] 苗晋锋[3] 陈伟[1] 韩先干[2] MAIERHABA·Wusiman;ZUO Jiakun;LYU Zhaoyang;JIANG Wei;CHEN Zhaoguo;ZHANG Xiuping;MIAO Jinfeng;CHEN Wei;HAN Xiangan(College of Animal Science and Technology,Tarim University,Alar 843300,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]塔里木大学动物科学与技术学院,阿拉尔843300 [2]中国农业科学院上海兽医研究所,上海200241 [3]南京农业大学,南京210095

出　　处：《中国动物传染病学报》2024年第3期141-148,共8页Chinese Journal of Animal Infectious Diseases

基　　金：上海市科技兴农重点攻关项目(2020-02-08-00-08-F01489);国家重点研发计划-政府间国际科技创新合作重点专项(2018YFE0102200);兵团财政科技计划项目-重点领域科技攻关计划(2020AB025)。

摘　　要：本研究对来自国内11个地区136份乳腺炎乳样开展了大肠杆菌的生物学特性研究。通过大肠杆菌phoA特异性鉴定引物对乳房炎乳样进行大肠杆菌分离与鉴定,采用K-B法对分离株进行药敏检测。建立3体系三重PCR检测方法,利用大肠杆菌9种毒力基因对分离株进行检测。通过PCR检测chuA、yjaA和TspE4.C2,对分离株进行进化分群鉴定。结果显示:136份乳腺炎乳样中共分离到61株大肠杆菌,阳性率为44.85%。分离株对头孢拉定、氨苄西林、四环素耐药率相对较高,分别为37.70%、32.78%和26.22%。分离株中毒力基因ompA和traT携带率较高(98.36%和75.40%),其次为CNF1(54.09%)、irp2(47.54%)和iucD(32.78%),sitD和chuA携带率较少(14.75%和13.11%),但ST2和hlyA基因均未检出。分群结果显示分离株以A群占(67.21%)和B1群(19.67%)为主,D群(8.19%)和B2群(4.91%)较少。本研究为奶牛乳腺炎源大肠杆菌的防控提供数据参考。To investigate the prevalence and characteristics of Escherichia coli mastitis of dairy cows,136 milk samples were collected from 11 provinces of China for bacterial isolation using PCR phoA amplication.The antibiotic sensitivity of all isolates was tested by the Kirby-Bauer disk diffusion method and their virulence genes were tested by the triplex PCR method.Then,phylogenetic analysis were performed for these isolates according to the genes of chuA,yjaA and TSPE4.C2.The results showed that 61(44.85%)E.coli isolates were obtained from 136 samples.These E.coli isolates had varying resistance to cefradine(37.70%),ampicillin(32.78%)and tetracycline(26.22%).The virulence genes were also detected at different rates,such as ompA(98.36%),traT(75.40%),CNF1(54.09%),irp2(47.54%),iucD(32.78%),sitD(14.75%)and chuA(13.11%).No isolates had ST2 and hlyA.In addition,grouping of these 61 E.coli isolates presented different results,such as 67.21%strains in group A,19.67%in group B1,8.19%in group D,and 4.91%in group B2.Groups A and B represented the main epidemic groups causing the clinical mastitis.Taken together,this study would provide a reference for the prevention and control of the E.coli cow mastitis.

关 键 词：奶牛乳腺炎 大肠杆菌 药物敏感性 毒力基因 进化分群 

分 类 号：S858.23[农业科学—临床兽医学]