敌敌畏和乐果农药对柞蚕幼虫中毒前后解毒酶活性的影响  

Effect of Dichlorvos and Dimethoate Pesticides on Detoxifying Enzyme Activities of the Tussah Larvae before and after Poisoning

作　　者：李晶涛[1] 宫田娇 张春杨 郭鸿儒 王乐天 杨淑芳[1] LI Jintao;GONG Tianjiao;ZHANG Chunyang;GUO Hongru;WANG Letian;YANG Shufang(Jilin Provincial Sericulture Research Institute,Jinlin 132012,China)

机构地区：[1]吉林省蚕业科学研究院,吉林吉林132012

出　　处：《北方蚕业》2024年第2期29-35,共7页North Sericulture

基　　金：财政部和农业农村部国家现代农业产业技术体系建设项目(CARS-18-SYZ02)。

摘　　要：通过研究敌敌畏和乐果两种农药对3龄和5龄柞蚕幼虫体内谷胱甘肽S-转移酶(GSTs)、乙酰胆碱酯酶(AChE)和羧酸酯酶(CES)活性的影响,结果表明:敌敌畏和乐果农药的半致死剂量LC_(50)72 h对3龄柞蚕幼虫血淋巴中3种酶的活性均有一定的抑制作用,其中敌敌畏的最大抑制率分别为31.27%、12.22%和13.38%,乐果的最大抑制率分别为10.22%、12.51%和9.17%;敌敌畏半致死剂量LC_(50)72 h和乐果给药剂量2 g/L条件下,对5龄柞蚕幼虫CES活性均有一定的激活作用,其中以敌敌畏对5龄柞蚕幼虫CES活性影响最大,血淋巴CES活性给药后1 h达最高,比对照组提高了21.37%;脂肪体、中肠、丝腺CES活性均在在给药后5 h达最高,比对照组分别提高了22.88%、21.29%和19.71%。This study investigated the effects of two pesticides,dichlorvos and dimethoate,on the activities of glutathione S-transferase(GSTs),acetylcholinesterase(AChE)and carboxylesterase(CES)in the 3 rd and 5 th instar of tussah larvae.The results showed that the semi-lethal dose LC_(50)72h of dichlorvos and dimethoate had certain inhibitory effects on the activities of the three enzymes in the hemolymph of 3 rd instar tussah larvae,with maximum inhibition rates of 31.27%,12.22%and 13.38%for dichlorvos,and 10.22%,12.51%and 9.17%for dimethoate,respectively.Under the conditions of semi-lethal dose of dichlorvos(LC_(50)72h)and dimethoate(2g/L),both pesticides had an activating effect on the CES activity of 5 th instar tussah larvae.Dichlorvos had the greatest influence on the CES activity of 5 th instar tussah larvae,with the hemolymph CES activity reaching its peak 1 hour after administration,showing a 21.37%increase compared to the control group.The CES activities in the fat body,midgut and silk gland all reached their peaks at 5 hours after administration,showing increases of 22.88%,21.29%and 19.71%,respectively,compared to the control group.

关 键 词：柞蚕 敌敌畏 乐果 解毒酶 

分 类 号：S884[农业科学—特种经济动物饲养]