利用差异转录组定位Paraburkholderia caffeinilytica CF1咖啡因降解功能基因  

Using differential transcriptome to located functional gene of Paraburkholderia caffeinilytica CF1

作　　者：陈森 高婕 王剑桥 高子晴[1] CHEN Sen;GAO Jie;WANG Jianqiao;GAO Ziqing(School of Biological Engineering,Dalian Polytechnic University,Dalian 116034,China;School of Environmental Engineering,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]广州大学环境科学与工程学院,广东广州510006

出　　处：《大连工业大学学报》2024年第4期251-256,共6页Journal of Dalian Polytechnic University

基　　金：广东省自然科学基金项目(2020A1515110189)。

摘　　要：实验室前期从茶园土壤中筛选到一株高效咖啡因降解菌CF1。为了寻找菌株CF1降解咖啡因的功能基因,对有无咖啡因添加条件下菌株CF1的转录组进行测序,并进行了差异转录组分析。结果显示,共得到4 871条Unigene序列,以菌株CF1的全基因组为模板,96.96%的序列可得到注释。组间差异基因分析结果显示,添加咖啡因条件下上调基因为123条,而下调基因为2 669条。在上调基因中,112条基因编码在染色体上,11条基因编码在巨型质粒上。通过Blast比对和关键酶酶活测定,证明了这11条编码在质粒上的基因与咖啡因降解功能直接相关,且聚集排布形成一条咖啡因降解基因簇。An efficient caffeine-degrading strain Paraburkholderia caffeinilytica CF1 isolated from a tea garden in China.To identify genes involved in caffeine degradation,a global transcription analysis of strain CF1 growing with and without caffeine addition were carried out and differential transcriptome analysis under the two conditions were conducted.The results showed that 4871 Unigene sequences were obtained and 96.96%of the obtained Unigene sequences could be annotated according to the whole genome sequence of strain CF1.The difference gene analysis between groups reveals that 123 genes were up-regulated and 2669 genes were down-regulated with caffeine addition.Among the up-regulated genes,112 genes were encoded on the chromosome and 11 genes arranged side by side were encoded on the giant plasmid.By analyzing the sequence alignment and activities of key enzymes,the redundant gene cluster containing the 11 up-regulated genes of caffeine degradation was elucidated.

关 键 词：咖啡因降解 转录组测序 差异转录组分析 

分 类 号：Q816[生物学—生物工程]