槐黄烷酮G抑制破骨细胞分化和减少骨丢失的机制研究  

Sophoraflavanone G inhibits RANKL-induced osteoclastogenesis and alleviates bone loss

作　　者：王民政 李泳瑜 刘为雯 张诗茵 梁梓炫 陈思敏[2] 郑泉鑫 黄立宇 钟露斌 江政晃 汤凯 WANG Minzheng;LI Yongyu;LIU Weiwen;ZHANG Shiyin;LIANG Xinxuan;CHEN Simin;ZHENG Quanxin;HUANG Liyu;ZHONG Lubin;JIANG Zhenghuang;TANG Kai(The Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine,Nanchang 330006;Guangzhou University of Chinese Medicine,Guangzhou 510405;Guangzhou Orthopedic Hospital,Guangzhou 510055;Guangzhou Yuehai Hospital,Guangzhou 510430;Longchuan County People’s Hospital,Heyuan 517399,Guangdong;The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]江西中医药大学附属医院,江西南昌330006 [2]广州中医药大学,广东广州510405 [3]广州市正骨医院,广东广州510055 [4]广州粤海医院,广东广州510430 [5]龙川县人民医院,广东河源517399 [6]广州中医药大学第一附属医院,广东广州510405

出　　处：《中国骨质疏松杂志》2024年第7期970-979,共10页Chinese Journal of Osteoporosis

基　　金：江西中医药管理局科技计划项目(2022A240);广州中医药大学大学生创新创业训练计划项目(202210572184)。

摘　　要：目的 通过体内与体外实验相结合探讨槐黄烷酮G抑制破骨细胞形成和分化,从而缓解骨丢失现象的作用机制研究。方法 通过CCK-8和TRAcP染色实验验证槐黄烷酮G是否具有抑制破骨细胞形成的作用;通过分子对接验证槐黄烷酮G与破骨细胞分化相关的特异性蛋白结合的亲和力大小,借助RT-PCR和Western blot实验探讨槐黄烷酮G抑制破骨细胞形成的作用机制;最后通过体内实验进一步探讨,以验证体外实验的准确性和可靠性。结果 槐黄烷酮G能够在安全浓度下抑制破骨细胞形成和分化,同时也与破骨细胞相关的特异性蛋白如NFATc1、CTSK、c-Fos、MMP9具有较高的亲和力,并能够降低上述基因和蛋白的相对表达水平,槐黄烷酮G也能够在15和30 min处有效降低MAPK信号通路中的ERK和JNK的磷酸化表达水平,体内实验证明槐黄烷酮G能够起到缓解骨丢失,改善骨小梁相关参数的作用。结论 槐黄烷酮G具有抑制破骨细胞形成和分化,缓解骨量减少的作用,从而起到抗骨质疏松症的作用。Objective To investigate the mechanism of Sophoraflavanone G in inhibiting osteoclast formation and differentiation,thereby alleviating bone loss,through a combination of in vivo and in vitro experiments.Methods The inhibitory effect of Sophoraflavanone G on osteoclast formation was verified with CCK-8 and TRAcP staining experiments.The affinity of Sophoraflavanone G binding to specific proteins related to osteoclast differentiation was validated with molecular docking.The mechanism of Sophoraflavanone G inhibition of osteoclast formation was explored using RT-PCR and Western blotting experiments.Finally,in vivo experiments were conducted to verify the accuracy and reliability of the in vitro experiments.Results Sophoraflavanone G inhibited the formation and differentiation of osteoclasts at safe concentrations.It had a high affinity with osteoclast-related specific proteins such as NFATc1,CTSK,c-Fos,and MMP9.It reduced the relative expression levels of these genes and proteins.Sophoraflavanone G effectively reduced the phosphorylation expression levels of ERK and JNK in the MAPK signaling pathway at 15 and 30 minutes.In vivo experiments showed that Sophoraflavanone G alleviated bone loss and improved trabecular related parameters.Conclusion Sophoraflavanone G has the ability to inhibit the formation and differentiation of osteoclasts,to alleviate bone loss,thereby playing a role in combating osteoporosis.

关 键 词：骨质疏松 破骨细胞 苦参 骨碎补 槐黄烷酮G 

分 类 号：R932[医药卫生—生药学] R589.5[医药卫生—药学]