5-ALA对干旱胁迫下沙棘叶绿素合成和光合作用的影响  

Effects of 5-ALA on Chlorophyll Synthesis and Photosynthesis of Hippophae rhamnoides under Drought Stress

作　　者：武亚琼 宣振国 WU Yaqiong;XUAN Zhenguo(Sangganhe Poplar High-yield Forest Experimental Bureau of Shanxi Province,Datong 037006,China)

机构地区：[1]山西省桑干河杨树丰产林实验局,山西大同037006

出　　处：《吉林林业科技》2024年第4期14-20,共7页Journal of Jilin Forestry Science and Technology

基　　金：山西省林业重点研发计划专项(LYZDYF2020-27)。

摘　　要：试验采用盆栽方式,设置无干旱胁迫对照、干旱胁迫对照和3个5-ALA质量浓度(40 mg·L^(-1)、80 mg·L^(-1)、120 mg·L^(-1))处理,探讨其对沙棘叶绿素合成和光合速率的影响。结果表明:干旱胁迫与无干旱胁迫相比降低了叶绿素含量,并显著降低了叶绿素合成的前体物质胆色素原、原卟啉IX和Mg-原卟啉Ⅸ含量;80 mg·L^(-1)的5-ALA会显著提高干旱胁迫下叶绿素合成的前体物质胆色素原、原卟啉IX和Mg-原卟啉Ⅸ含量,处理后第30天、第45天和第60天会显著提高叶片内叶绿素含量;40 mg·L^(-1)5-ALA在处理后第60天不能显著提高干旱胁迫下叶绿素、胆色素原、原卟啉IX和Mg-原卟啉Ⅸ含量;干旱胁迫降低了沙棘叶片净光合速率和Rubisco活性,80 mg·L^(-1)5-ALA可以显著提高干旱胁迫下净光合速率和Rubisco活性;5-ALA质量浓度为120 mg·L^(-1)和80 mg·L^(-1)时干旱胁迫下叶绿素含量和净光合速率差异不显著。综上所述,80 mg·L^(-1)的5-ALA更适于促进干旱胁迫下沙棘叶绿素合成和提高其光合能力。The pot experiment was conducted to investigate effects of 5-ALA on chlorophyll synthesis and photosynthetic rate of Hippophae rhamnoides under drought stress by setting no drought stress control,drought stress control and three 5-ALA concentrations(40 mg·L^(-1),80 mg·L^(-1) and 120 mg·L^(-1)).The results showed that the chlorophyll content in the leaf under drought stress was lower than that under no drought stress,and the contents of precursor substances(porphobilinogen,protoporphyrin IX,and Mg-protoporphyrin IX)for chlorophyll synthesis under drought stress were significantly lower than those under no drought stress.The contents of precursor substances(porphobilinogen,protoporphyrin IX,and Mg-protoporphyrin IX)for chlorophyll synthesis under drought stress increased significantly with the 5-ALA concentration of 80 mg·L^(-1),and the chlorophyll content increased significantly on the 30th day,45th day and 60th day after treatment.The contents of chlorophyll,porphobilinogen,protoporphyrin IX,and Mg-protoporphyrin IX under drought stress did not increase significantly with the 5-ALA concentration of 40 mg·L^(-1) on the 60th day after treatment.The net photosynthetic rate of the leaf and Rubisco activity decreased under drought stress,and those increased significantly with the 5-ALA concentration of 80 mg·L^(-1).The chlorophyll content and net photosynthetic rate under drought stress with 5-ALA concentration of 120 mg·L^(-1) were not significantly different from those with 5-ALA concentration of 80 mg·L^(-1).In summary,the chlorophyll synthesis and photosynthetic capacity were better with the 5-ALA concentration of 80 mg·L^(-1) under drought stress.

关 键 词：5-ALA 干旱胁迫 沙棘 叶绿素 净光合速率 

分 类 号：S793.6[农业科学—林木遗传育种]