Modelling the development of biological structures displaying longitudinal geometries in vitro:culturing pluripotent stem cells on plasma-treated,growth factor-coupled polycaprolactone fibres  

作　　者：Badwi B.Boumelhem Stuart T.Fraser Syamak Farajikhah Rachel A.Shparberg Michael B.Morris Marcela M.M.Bilek Anyu Zhang Behnam Akhavan Simon Fleming Maryanne Large 

机构地区：[1]School of Physics,Faculty of Science,University of Sydney,Australia [2]Sydney Nano Institute,University of Sydney,Australia [3]School of Chemical and Biomolecular Engineering,University of Sydney,Australia [4]School of Medical Sciences,Faculty of Medicine and Health,University of Sydney,Australia [5]School of Biomedical Engineering,Faculty of Engineering,University of Sydney,Australia [6]School of Engineering,University of Newcastle,Callaghan,NSW 2308,Australia [7]Hunter Medical Research Institute(HMRI),Precision Medicine Program,New Lambton Heights NSW 2305,Australia

出　　处：《Engineered Regeneration》2024年第1期124-138,共15页再生工程（英文）

基　　金：supported by the Australian Research Council Laureate and Discovery fundings[FL190100216,DP190103507 and DE210100662];the University of Sydney School of Physics“Grand Challenge”program.

摘　　要：Many biological structures such as nerves,blood and lymphatic vessels,and muscle fibres exhibit longitudinal ge-ometries with distinct cell types extending along both the length and width of internal linear axes.Modelling these three-dimensional structures in vitro is challenging:the best-defined stem-cell differentiation systems are mono-layer cultures or organoids using pluripotent stem cells.Pluripotent stem cells can differentiate into functionally mature cells depending on the signals received,holding great promise for regenerative medicine.However,the integration of in vitro differentiated cell types into diseased tissue remains a challenge.Engineered scaffolds can bridge this gap if the appropriate signalling systems are incorporated into the scaffold.Here,we have taken a biomimicry approach to generate longitudinal structures in vitro.In this approach,mouse embryonic stem cells are directed to differentiate to specific cell types on the surface of polycaprolactone(PCL)fibres treated by plasma-immersion ion implantation and to which with lineage-specifying molecules have been covalently im-mobilised.We demonstrate the simplicity and utility of our method for efficiently generating high yields of the following cell types from these pluripotent stem cells:neurons,vascular endothelial cells,osteoclasts,adipocytes,and cells of the erythroid,myeloid,and lymphoid lineages.Strategically arranged plasma-treated scaffolds with differentiated cell types could ultimately serve as a means for the repair or treatment of diseased or damaged tissue.

关 键 词：Pluripotent stem cells Lineage commitment Growth factors Biomaterial engineering Plasma-immersion ion implantation Covalent biomolecule attachment POLYCAPROLACTONE 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]