Phenotype-Directed DNA Nanomachine for in Situ Analysis of Stem Cell-like Subpopulation in Breast Cancer  

作　　者：Ya Cao Huiru Mao Lingjun Sha Qi Liu Bing Han Jing Zhao Genxi Li 

机构地区：[1]Center for Molecular Recognition and Biosensing,Shanghai Engineering Research Center of Organ Repair,School of Life Sciences,Shanghai University,Shanghai 200444 [2]State Key Laboratory of Analytical Chemistry for Life Science,School of Life Sciences,Nanjing University,Nanjing 210023

出　　处：《CCS Chemistry》2024年第4期1034-1046,共13页中国化学会会刊（英文）

基　　金：the National Natural Science Foundation of China(grant nos.81972799 and 81871449);the Natural Science Foundation of Shanghai(grant no.23ZR1421400).

摘　　要：The cancer stem cell hypothesis provides a basis for prediction of the recurrence and risk of metastasis in breast cancer.However,the unique expression pattern of stemness markers and the presence of nonstem-like cancer cells with varied phenotypes have brought great challenges to the characterization of breast cancer stem cells.To address these challenges,a phenotype-directed DNA nanomachine has been designed for high-accuracy labeling and in situ analysis of the stem cell-like subpopulation in breast cancer.The key for the design is to use cell surfaceanchored inputs to activate the nanomachine,which undergoes different branch migration pathways such that the signal strand can only be brought onto the cancer cells having the stem cell-like phenotype.Highly sensitive determination and single-step isolation of the stem cell-like subpopulation were achieved by incorporating functional groups into the signal strand such that the nanomachine was successfully applied in a tumor-bearing mouse model.Overall,the approach provides for a substantial improvement in capability for the analysis of the breast cancer stem cell-like subpopulation,and it is expected that the new approach will advance the use of DNA nanomachines in cancer-related studies.

关 键 词：breast cancer DNA nanomachine stem cell-like subpopulation electrochemical determination single-step isolation phenotypic analysis 

分 类 号：O646[理学—物理化学]