Combatting Pathogenic Bacteria with Synthetic Immunotherapeutics from Chitosan:Antibody Recruiting at Engineered Bacterial Surface with Peptidoglycan Analogs  

作　　者：Yuan Xu Shihao Song Hongwei Duan Yinyue Deng Xue-Wei Liu 

机构地区：[1]School of Chemistry,Chemical Engineering and Biotechnology,Nanyang Technological University,Singapore 637371 [2]Key Laboratory of Tropical Biological Resources of Ministry of Education,School of Pharmaceutical Sciences,Hainan University,Haikou 570228 [3]School of Pharmaceutical Sciences(Shenzhen),Shenzhen Campus of Sun Yat-sen University,Sun Yat-sen University,Shenzhen 518107

出　　处：《CCS Chemistry》2024年第6期1448-1457,共10页中国化学会会刊（英文）

基　　金：the Ministry of Education(grant nos.MOET2EP30120-0007 and MOE2018-T2-2-128);the National Research Foundation(grant no.NRF-CRP22-2019-0002);Agency for Science,Technology and Research(A*STAR);Singapore(grant no.A20E5c0087);the National Natural Science Foundation of China(grant no.32300033);the Scientific Research Foundation of Hainan University(grant nos.KYQD(ZR)-22173 and KYQD(ZR)-23006).

摘　　要：The emergence of microbial drug resistance,coupled with the paucity of new antibiotics poses an impending threat to public health.In this work,we drew inspiration from synthetic peptidoglycan oligomers and successfully constructed antibody-recruiting peptidoglycan analogs,2a-d,with excellent safety profiles and high efficiencies in recruiting antibodies across different conditions.Further,we demonstrated that these peptidoglycan analogs could be readily incorporated into bacteria cell walls,whereby both simple monoclonal and pooled human serum antibodies effectively congregated to the surfaceengineered bacteria,leading to the complete extermination of the engineered bacterial cells both in vitro and in vivo.Our peptidoglycan analog agents for recruiting endogenous antibodies to combat pathogenic bacteria enable further development of promising broad-spectrum immunotherapeutics.

关 键 词：CHITOSAN bacterial infection endogenous antibodies bacterial surface engineering peptidoglycan analogs 

分 类 号：O62[理学—有机化学]