A Fur-regulated type Ⅵ secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis  

作　　者：Yuxin Zuo Changfu Li Danyang Yu Kenan Wang Yuqi Liu Zhiyan Wei Yantao Yang Yao Wang Xihui Shen Lingfang Zhu 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,Shaanxi Key Laboratory of Agricultural and Environmental Microbiology,College of Life Sciences,Northwest A&F University,Yangling 712100,Shaanxi,China

出　　处：《Stress Biology》2023年第1期20-34,共15页逆境生物学（英文）

基　　金：supported by grants of the National Key R&D Program of China(Grants 2018YFA0901200);National Natural Science Foundation of China(Grants 31725003,31970114,32100034,and 32100149);Scientific Startup Foundation for Doctors of Northwest A and F University(Z1090122001 and Z1090122002);the China Postdoctoral Science Foundation(Grant 2020 M673501);Young Talent Support Program of Shaanxi Province University(20220206).

摘　　要：The type Ⅵ secretion system(T6SS)is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria,host organisms,and the environment.Yersinia pseudotuber-culosis T6SS4 was recently reported to be involved in manganese acquisition;however,the underlying regulatory mechanism still remains unclear.In this study,we discovered that T6SS4 is regulated by ferric uptake regulator(Fur)in response to manganese ions(Mn^(2+)),and this negative regulation of Fur was proceeded by specifically recogniz-ing the promoter region of T6SS4 in Y.pseudotuberculosis.Furthermore,T6SS4 is induced by low Mn^(2+)and oxidative stress conditions via Fur,acting as a Mn^(2+)-responsive transcriptional regulator to maintain intracellular manganese homeostasis,which plays important role in the transport of Mn^(2+)for survival under oxidative stress.Our results pro-vide evidence that T6SS4 can enhance the oxidative stress resistance and virulence for Y.pseudotuberculosis.This study provides new insights into the regulation of T6SS4 via the Mn^(2+)-dependent transcriptional regulator Fur,and expands our knowledge of the regulatory mechanisms and functions of T6SS from Y.pseudotuberculosis.

关 键 词：TypeⅥsecretion system FUR MANGANESE Oxidative stress VIRULENCE 

分 类 号：Q93[生物学—微生物学]