VmPacC-mediated pH regulation of Valsa mali confers to host acidification identified by comparative proteomics analysis  

作　　者：Liangsheng Xu Hailong Liu Shan Zhu Yangguang Meng Yinghao Wang Jianyu Li Feiran Zhang Lili Huang 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,Yangling,Shaanxi 712100,China

出　　处：《Stress Biology》2023年第1期201-218,共18页逆境生物学（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(Grant No.31772115 and U1903206).

摘　　要：Apple valsa canker caused by the Ascomycete fungus Valsa mali is one of the most serious diseases of apple,resulting in huge economic losses in the apple-growing area of China.Previous study found that the pathogen could acidify the infected tissues to make lower ambient pH(from 6.0 to 3.5)for their successfully colonization.The pH signaling transcription factor VmPacC is required for acidification of its environment and for full virulence in V.mali.It is known that the functional cooperation of proteins secreted by V.mali plays pivotal role in its successful colonization of host plants.In this study,we used tandem mass tag(TMT)labeling coupled with LC-MS/MS-based quantitative proteomics to analyze the VmPacC-mediated pH regulation in V.mali,focusing on differentially expressed proteins(DEPs).We identified 222 DEPs specific to VmPacC deletion,and 921 DEPs specific to different pH conditions(pH 6.0 and 3.4).Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analyses indicated that these DEPs were mainly involved in pathways associated with carbon metabolism,biosynthesis of antibiotics,citrate cycle(TCA cycle),glycolysis/gluconeogenesis,glutathione metabolism,ribosomes,and pentose phosphate pathways.Additionally,we identified 119 DEPs that were shared among the VmPacC deletion mutant and different pH conditions,which were mainly related to energy metabolism pathways,providing the energy required for the hyphal growth and responses to environmental stresses.A protein-protein interaction(PPI)network analysis indicated that most of the shared proteins were mapped to an interaction network with a medium confidence score of 0.4.Notably,one uncharacterized protein(KUI69106.1),and two known proteins(heat shock protein 60(KUI73579.1),aspartate aminotransferase(KUI73864.1))located in the core of the network were highly connected(with≥38 directed edges)with the other shared DEPs.Our results suggest that VmPacC participates in the pathogen’s regulation to ambient pH through the regulation of energy

关 键 词：Valsa mali TMT PACC Proteomics Differentially expressed protein 

分 类 号：S661.1[农业科学—果树学]