Molecular characterization and pathogenicity of an infectious clone of tomato leaf curl New Delhi virus isolate infecting Cucumis melo  

作　　者：Yuzhen Mei Lingmin Cai Yaqin Wang Fangfang Li Xiuling Yang Jinghua Yang Xueping Zhou 

机构地区：[1]State Key Laboratory of Rice Biology,Institute of Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang,China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China [3]Laboratory of Germplasm Innovation and Molecular Breeding,Institute of Vegetable Science,Zhejiang University,Hangzhou 310058,China [4]Yazhou Bay Science and Technology City,Hainan Institute,Zhejiang University,Sanya 572025,China

出　　处：《Stress Biology》2023年第1期546-554,共9页逆境生物学（英文）

基　　金：supported by the National Key Research and Development Program of China(2021YFD1400400);the National Natural Science Foundation of China(31930089).

摘　　要：Tomato leaf curl New Delhi virus(ToLCNDV)is a member of the genus Begomovirus,and causes devastating disease in the world.In recent years,ToLCNDV was rapidly spreading in China and induces severe economic losses in agriculture.In this study,we sequenced and characterized the complete genome of ToLCNDV isolates from melon plants showing leaf curling and stunting symptoms in Jiangsu Province of China.We constructed a full-length infectious cDNA clone of ToLCNDV,which could induce systemic infection with typical symptoms in Nicotiana benthamiana,Cit-rullus melo,and Citrullus lanatus plants through agrobacterium-mediated inoculation.Further experimental evidence demonstrated that the virions produced in plants infected with the infectious clone of ToLCNDV are biologically active and sap-transmissible.We also evaluated the resistance of commercial melon cultivars to ToLCNDV and found all testing melon cultivars were susceptible to ToLCNDV.Collectively,the reverse genetic system developed herein will facilitate further research on biological functions of proteins encoded by ToLCNDV and plant-ToLCNDV interactions,which might provide new insights into breeding resistance germplasm in crops.

关 键 词：BEGOMOVIRUS Tomato leaf curl New Delhi virus Infectious clone 

分 类 号：S43[农业科学—农业昆虫与害虫防治]