基于网络药理学和裸鼠实验探讨芪术增力方治疗癌因性疲乏的作用机制  

Exploring the Mechanism of Qizhu Zengli Fang in Treating Cancer-Related Fatigue Based on Network Pharmacology and Nude Mouse Experiments

作　　者：卢茜[1] 罗溢昌 许双洁 王云启[3] LU Xi;LUO Yichang;XU Shuangjie;WANG Yunqi(Guangzhou University of Chinese Medicine,Guangzhou 510000,China;The Affiliated Traditional Chinese Medicine Hospital of Guangzhou Medical University,Guangzhou 510000,China;Hunan Cancer Hospital,Changsha 410013,China)

机构地区：[1]广州中医药大学,广州510000 [2]广州医科大学附属中医医院,广州510000 [3]湖南省肿瘤医院,长沙410013

出　　处：《中国中医基础医学杂志》2024年第7期1173-1177,共5页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：湖南省中医药管理局资助项目(202033)。

摘　　要：目的观察芪术增力方对裸鼠癌因性疲乏的影响,探讨其可能的作用机制。方法在TCMSP平台搜索芪术增力方活性成分,通过Uniprot数据库转化为相关靶点;在Gene Cards数据库、OMIM数据库、DrugBank数据库搜索癌因性疲乏相关靶点,通过Jvenn平台绘制药物、疾病靶点交集图,String平台制作蛋白质互作网络(protein-protein interaction networks,PPI),Metascape平台进行GO和KEGG富集分析,选取癌症蛋白聚糖(proteoglycans in cancer)信号通路进行研究。将25只裸鼠随机分为空白组、模型组、中药组、化疗组(顺铂)及地塞米松组。构建癌因性疲乏模型,干预3周后,ELISA法检测裸鼠瘤体内磷脂酰肌酶聚糖受体(glypican,GPC)1、GPC5、多配体蛋白聚糖(syndecan,SDC)2、丝甘蛋白聚糖(serglycin gene,SRGN)表达;观察光镜下各组裸鼠瘤体组织的病理形态。结果与模型组比较,中药组及化疗组的GPC1水平显著降低(P<0.01);中药组SDC2水平降低(P<0.05);中药组、化疗组、地塞米松组瘤体内SRGN水平均降低(P<0.05)。结论芪术增力方通过降低裸鼠瘤体内GPC1、SDC2、SRGN水平达到缓解疲乏的效果,其机制可能与抑制肿瘤生长、转移有关。Objective By observing the effects of Qizhu Zengli Fang on cancer-related fatigue(CRF)in nude mice,to explore its therapeutic effects.Methods The active ingredients of Qizhu Zengli Fang were searched on the TCMSP platform,and converted into relevant targets through the Uniprot database.The targets related to CRF were searched in the GeneCards database,OMIM database,and DrugBank database.Then,the Jvenn platform was used to draw the intersection map of drugs and disease targets.The String platform was used to create a protein-protein interaction networks(PPI),and the Metascape platform was used to create GO and KEGG enrichment analysis.Among them,selected the proteoglycans in cancer signaling pathway for research.25 nude mice were randomly divided into blank group,model group,Chinese medicine group,chemotherapy group(cisplatin)and dexamethasone group.The CRF model was constructed.After 3 weeks of intervention,GPC1,GPC5,SDC2,and SRGN were detected by ELISA.Observed the pathological morphology of tumor tissue in nude mice in each group under light microscope.Results Compared with model group,the expression of GPC1 in the Chinese medicine group and the chemotherapy group was significantly decreased(P<0.01).The expression of SDC2 were decreased in Chinese medicine group(P<0.05).The SRGN of the Chinese medicine group,chemotherapy group,and dexamethasone group decreased(P<0.05).Conclusion Qizhu Zengli Fang can relieve fatigue by decreased the expression of GPC1,SDC2,and SRGN in tumors of nude mice,and its mechanism was speculated to be related to the inhibition of tumor growth and metastasis.

关 键 词：网络药理学 癌因性疲乏 芪术增力方 肺复康方 蛋白聚糖 

分 类 号：R285.5[医药卫生—中药学]