富血小板血浆促进创面愈合的细胞和分子机制  

Cellular and molecular mechanisms of platelet-rich plasma in promoting wound healing

作　　者：龙晨艳 程飚 田举 Long Chenyan;Cheng Biao;Tian Ju(First Clinical Medical College of Guangdong Medical University,Zhanjiang 524000,Guangdong Province,China;Department of Burns,Plastic Surgery,and Aesthetic Medicine,People’s Hospital of Zhongshan City,Guangdong Medical University,Zhongshan 528400,Guangdong Province,China;Department of Burns and Plastic Surgery,General Hospital of Southern Theater Command,Guangzhou 510030,Guangdong Province,China)

机构地区：[1]广东医科大学第一临床医学院,广东省湛江市524000 [2]广东医科大学中山市人民医院烧伤整形美容科,广东省中山市528400 [3]南部战区总医院烧伤整形科,广东省广州市510030

出　　处：《中国组织工程研究》2025年第13期2793-2801,共9页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(82172223,82372531),项目负责人:程飚;广东省中医药局科研项目(20241357),项目负责人:田举。

摘　　要：背景:尽管富血小板血浆在促进创面愈合方面显示出治疗潜力,但其临床应用仍需要进一步的研究和验证。此外,对于富血小板血浆作用的机制仍存在一些争议和不确定性。目的:通过对富血小板血浆促进创面愈合的机制进行概述和分析,以增进对该治疗方法的理解,为未来的基础研究和临床应用提供有益的指导。方法:在中国知网、PubMed数据库检索2000年1月至2023年12月发表的相关文献。英文检索词为“platelet rich plasma,PRP,wound,wound healing,refractory wounds,chronic wounds,cellular and molecular mechanisms,signaling pathways,regenerative medicine”,中文检索词为“富血小板血浆,PRP,创面,伤口,创面愈合,难愈性创面,慢性创面,细胞和分子机制,信号通路,再生医学”。根据纳入和排除标准,最终对80篇文献进行综述。结果与结论:①富血小板血浆在创面愈合中具有许多潜在的优势,它能够提供丰富的生长因子和细胞因子等生物活性物质;②富血小板血浆可以调节参与创面愈合的各种细胞类型的功能,包括表皮细胞、血管内皮细胞、成纤维细胞、干细胞和炎症细胞等;③在富血小板血浆疗法中,细胞外囊泡、线粒体对于创面愈合可能扮演着重要角色;④负载富血小板血浆水凝胶可广泛用于增强组织再生和作为药物传递的功能性载体;⑤富血小板血浆对创面细胞外基质合成和降解起调节作用,从而影响伤口愈合过程中基质重建;⑥富血小板血浆可能通过动态调节多种信号通路和上皮间质转化来影响组织修复与再生,从而为创面愈合的研究提供了全新的视角。BACKGROUND:Although platelet-rich plasma has shown potential in promoting wound healing,further research and validation are still needed for its clinical application.Additionally,there are still some controversies and uncertainties regarding the action mechanisms of platelet-rich plasma.OBJECTIVE:To provide an overview and analysis of the mechanisms of platelet-rich plasma in promoting wound healing,in order to enhance understanding of this treatment method and provide useful guidance for future basic research and clinical applications.METHODS:Relevant literature published from January 2000 to December 2023 was retrieved from the China National Knowledge Infrastructure(CNKI)and PubMed databases.The English and Chinese search terms included“platelet rich plasma,PRP,wound,wound healing,refractory wounds,chronic wounds,cellular and molecular mechanisms,signaling pathways,regenerative medicine.”A total of 80 articles were reviewed based on inclusion and exclusion criteria.RESULTS AND CONCLUSION:(1)Platelet-rich plasma has many potential advantages in wound healing as it can provide abundant bioactive substances such as growth factors and cytokines.(2)Platelet-rich plasma regulates the function of various cell types involved in wound healing,including epidermal cells,vascular endothelial cells,fibroblasts,stem cells,and inflammatory cells.(3)In platelet-rich plasma therapy,extracellular vesicles and mitochondria may play important roles in wound healing.(4)Platelet-rich plasma hydrogels can be widely used to enhance tissue regeneration and serve as functional carriers for drug delivery.(5)Platelet-rich plasma regulates the synthesis and degradation of the extracellular matrix,affecting matrix remodeling during the wound healing process.(6)Platelet-rich plasma may influence tissue repair and regeneration by dynamically regulating multiple signaling pathways and the epithelial-mesenchymal transition,providing a new perspective for research in this field.

关 键 词：富血小板血浆 生长因子 创面愈合 细胞和分子机制 信号通路 再生医学 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R62