富血小板血浆干预细胞自噬和凋亡治疗骨关节炎  

Platelet-rich plasma intervenes in chondrocyte autophagy and apoptosis for treatment of osteoarthritis

作　　者：王耀敏 张克凡 王德宁 任强[1] 李健[1] 石辉[1] Wang Yaomin;Zhang Kefan;Wang Dening;Ren Qiang;Li Jian;Shi Hui(Binzhou Medical University Hospital,Binzhou 256600,Shandong Province,China;Binzhou Medical University,Binzhou 264003,Shandong Province,China)

机构地区：[1]滨州医学院附属医院,山东省滨州市256600 [2]滨州医学院,山东省滨州市264003

出　　处：《中国组织工程研究》2025年第13期2802-2811,共10页Chinese Journal of Tissue Engineering Research

基　　金：山东省省级临床重点专科学科建设项目(SLCZDZK-0302),项目负责人:石辉;滨州医学院徐荣祥再生医学发展计划项目(BY2019XRX04),项目负责人:任强;山东省医药卫生科技发展计划(2017WS040),项目负责人:任强;山东省医药卫生科技项目(202304070630),项目负责人:李健。

摘　　要：背景:富血小板血浆通过调节自噬和凋亡细胞因子、信号转导通路等方面在干预骨关节炎发展过程中发挥了重要作用。目的:总结近年来富血小板血浆在骨关节炎中发挥作用的细胞因子与信号通路,以及与软骨细胞自噬和凋亡的相关性,为未来治疗骨关节炎提供有效的靶点。方法:在中国知网、万方数据库、维普、PubMed、Web of Science和Medline数据库进行文献检索,以“富血小板血浆,软骨细胞,细胞凋亡,细胞自噬,骨关节炎,细胞因子,信号通路”作为中文检索词,以“platelet-rich plasma,chondrocyte,apoptosis,autophagy,osteoarthritis,cytokines,signaling pathway”作为英文检索词,对最终纳入的66篇文献进行了系统性的总结和归纳。结果与结论:现有研究显示富血小板血浆能够通过多种途径促进软骨修复,助力骨组织愈合,其主要分为3个方面:①富血小板血浆参与调控了微自噬小体的延伸、闭合与成熟,并在特定条件下促进软骨细胞巨自噬和分子伴侣介导的细胞自噬,促进LC3Ⅱ/Ⅰ、Beclin1等自噬相关因子的表达,抑制P62/SQSTM1的表达,目前尚未有明确的研究直接探讨富血小板血浆对热休克蛋白的具体作用,未来在这一领域值得进一步研究;②富血小板血浆释放的各类生长因子抑制促凋亡因子Caspase、白细胞介素1β、肿瘤坏死因子α的表达,促进抗凋亡因子Bcl-2的表达,阻止软骨细胞凋亡和变性;③富血小板血浆通过激活PI3K/AKT/mTOR信号通路、NF-κB信号转导通路、死亡受体通路、线粒体应激通路等途径,抑制Bax和Caspase的表达,阻止细胞色素c的释放,从而抑制软骨细胞死亡和坏死性凋亡。综合而言,富血小板血浆促进软骨修复、支持软骨再生及发挥抗炎作用,其在软骨细胞中实现生物效应通常依赖于细胞自噬和凋亡相关细胞因子及信号通路的调控。BACKGROUND:In the process of intervening in the development of osteoarthritis,platelet-rich plasma plays an important role by intervening in autophagy,apoptotic cytokines and signal transduction pathways.OBJECTIVE:To summarize the structure of cytokines and signaling pathways involved in the diagnosis of osteoarthritis by platelet-rich plasma in recent years,as well as its correlation with chondrocyte apoptosis and autophagy,in order to provide effective targets for the future treatment of osteoarthritis.METHODS:Literature search was conducted in CNKI,WanFang Data,VIP,PubMed,Web of Science,and Medline databases using“platelet-rich plasma,chondrocyte,apoptosis,autophagy,osteoarthritis,cytokines,signaling pathway”as Chinese and English search terms.A systematic summary and induction were made for the 66 included articles.RESULTS AND CONCLUSION:Current research has shown that platelet-rich plasma can promote cartilage repair and assist bone tissue healing through various pathways,mainly divided into three aspects:(1)Platelet-rich plasma regulates the extension,closure,and maturation of microautophagosomes,promotes chondrocyte megaautophagy and molecular chaperone-mediated cell autophagy under specific conditions,enhances the expression of autophagy-related factors such as LC3II/I and Beclin1,inhibits the expression of P62/SQSTM1.Currently,there is no clear research directly exploring the specific effect of plateletrich plasma on heat shock proteins,and further research is needed in this field in the future.(2)The various growth factors released by platelet-rich plasma inhibit the expression of pro-apoptotic factors Caspase,interleukin-1β,and tumor necrosis factor alpha,promote the expression of anti-apoptotic factor Bcl-2,and prevent chondrocyte apoptosis and degeneration.(3)By activating the PI3K/AKT/mTOR signaling pathway,NF-κB signal transduction pathway,death receptor pathway,mitochondrial stress pathway and other pathways,platelet-rich plasma inhibits the expression of Bax and Caspase,and prevents the rel

关 键 词：富血小板血浆 软骨细胞 细胞凋亡 细胞自噬 骨关节炎 细胞因子 信号通路 

分 类 号：R459.9[医药卫生—治疗学] R332[医药卫生—临床医学] R684.3