基于月桂酰肌氨酸异丙酯为油相的高负载量槲皮素微乳的研究  

Study of quercetin-loaded microemulsion with high loading capacity based on isopropyl lauroyl sarcosine as oil phase

作　　者：王强 徐永林 刁岩 Qiang Wang;Yonglin Xu;Yan Diao(Collaborative Innovation Center of Tissue Repair Material of Sichuan Province,College of Life Sciences,China West Normal University,Nanchong,Sichuan 637009,China)

机构地区：[1]西华师范大学生命科学学院组织修复材料工程技术协同创新中心,四川南充637009

出　　处：《日用化学工业（中英文）》2024年第7期821-827,共7页China Surfactant Detergent & Cosmetics

基　　金：四川省自然科学基金(2022NSFSC1657);西华师范大学博士启动基金(18Q070)。

摘　　要：槲皮素在清除自由基以及皮肤保护等领域具有重要的应用前景,然而其存在水溶性低等缺陷,造成其载体开发受到一定程度的限制。前期研究发现,槲皮素在月桂酰肌氨酸异丙酯中的溶解度可达(83.30±2.65)mg/g,并远高于其他油脂,因此通过将具有较高槲皮素增溶性能的月桂酰肌氨酸异丙酯作为油相,采用低能乳化法构建槲皮素微乳体系,并对该体系的理化性质进行评价。结果表明,槲皮素微乳体系呈现圆整规整的球形,成型性好且分散较为均匀,其平均粒径为(38.6±0.5)nm,PDI值为0.18±0.07,Zeta电位为(-28.16±1.02)mV,pH值为6.23±0.18,同时测得体系中槲皮素的包封率为98.85%±2.36%,负载量为3.24%±0.12%。此外,构建的槲皮素微乳体系还属于非牛顿流体中的假塑性流体,并可表现出更多的弹性特征。上述结果表明月桂酰肌氨酸异丙酯作为微乳的分散相具有显著提高槲皮素溶解度和负载量的潜力,这对于提高槲皮素的功效以及减少微乳体系的用量或使用频率均具有重要意义。Quercetin is well known for its significant scavenging properties on oxidizing species,and can play a protective role against ultraviolet radiation-induced damage,thus improving skin condition and preventing skin aging.However,its low solubility in water is the main reason of its formulation difficulties.To overcome these problems,a proper and effective drug delivery system development is essential.Moreover,previous studies found that the solubility of quercetin in isopropyl lauroyl sarcosine could reach a maximum of(83.30±2.65)mg/g,which was much higher than other oils.Therefore,in this study,quercetin-loaded microemulsion was prepared by low energy emulsification method using isopropyl lauroyl sarcosine as the oil phase due to its good solubilizing capacity,and then some physicochemical properties(i.e.,microscopic morphology,droplet size,pH,encapsulation efficiency,loading capacity,etc.)of the microemulsion system were evaluated.The microemulsion is found to be spherical in shape with an uniform size distribution,and the average droplet size,PDI,Zeta potential and pH value are measured to be(38.6±0.5)nm,0.18±0.07,(-28.16±1.02)mV and 6.23±0.18,respectively.Meanwhile,the encapsulation efficiency and loading capacity of quercetin in the microemulsion system are calculated to be 98.85%±2.36%and 3.24%±0.12%,respectively.Additionally,it can be found that the microemulsion system presented non-Newtonian pseudoplastic fluid and predominantly elastic behaviors.The above results indicate that the microemulsion system comprising isopropyl lauroyl sarcosine as the dispersion phase has the potential to effectively improve the solubility and loading capacity of quercetin,which is of great significance for improving the efficacy of quercetin and reducing the used amount and frequency of the microemulsion system.

关 键 词：月桂酰肌氨酸异丙酯 槲皮素 微乳 高负载量 

分 类 号：TQ658[化学工程—精细化工]