蚊虫复合组DNA芯片鉴定技术的初步研究  

Preliminary Study on the Identification Technology of Mosquito Complexes Using DNA Chip

作　　者：林诗颖 彭晓丽 曾志昌 黄恩炯 LIN Shi-Ying;PENG Xiao-Li;ZENG Zhi-Chang;HUANG En-Jiong(Fuzhou International Travel Health Care Center,Fuzhou 350001;The School of Public Health,Fujian Medical University,Fuzhou 350122;Comprehensive Technology Service Center,Rongcheng Customs,Fuqing 350301)

机构地区：[1]福州国际旅行卫生保健中心,福州350001 [2]福建医科大学公共卫生学院,福州350122 [3]榕城海关综合技术服务中心,福清350301

出　　处：《中国口岸科学技术》2024年第6期86-95,共10页China Port Science and Technology

基　　金：福建省对外科技合作项目(2020I0031);福州海关科研项目(FK2021-05)。

摘　　要：本研究以ITS2基因为分子标记设计杂交探针,制作DNA芯片鉴定杂鳞库蚊复合组、白纹伊蚊亚组和尖音库蚊复合组中的8种/亚种蚊虫。结果表明,以ITS2基因为分子标记设计的DNA芯片可用于3个复合组(亚组)组间蚊虫以及杂鳞库蚊复合组的组内蚊虫鉴定,且白纹伊蚊亚组探针、尖音库蚊复合组探针、杂鳞库蚊复合组探针、三带喙库蚊探针、伪杂鳞库蚊探针、致倦库蚊探针、尖音库蚊探针具有良好的专一性和特异性。现场测试表明,该DNA芯片可应用于识别不同发育阶段和不同地区的同种蚊虫,但对尖音库蚊和致倦库蚊表现出非专一性杂合。This study employed the ITS2 gene as a molecular marker for the design of hybridization probes,creating a DNA chip for the identification of eight species/subspecies within the Cules vishnui complex,Aedes albopictus subgroup,and Cx.pipiens com-plex.The findings indicate that the DNA chip,developed with the ITS2 gene as the molecular marker,is suitable for the inter-complex(subgroup)and intra-complex identification of mosquitoes,including those within the Cx.vishnui complex.The probes specific to the Ae.albopictus subgroup,Cx.pipiens complex,Cx.vishnui complex,Cx.tritaeniorhynchus,Cx.pseudovishnui,Cx.quinquefasciatus,and Cx.pipiens displayed high specificity and selectivity.Field assessments have confirmed the applicability of the DNA chip in iden-tifying conspecific mosquitoes at different developmental stages and from diverse regions.However,the chip exhibited non-specific hybridization for Cx.pipiens and Cx.quinquefasciatus.

关 键 词：蚊虫 复合组(亚组) DNA芯片 鉴定 

分 类 号：R384.1[医药卫生—医学寄生虫学]