5基因编辑猪-恒河猴异种心脏移植实验报道  

Report of 5 gene-edited pig-rhesus monkey heterotopic heart xenotransplantation experiment

作　　者：张根 王欢[1] 管玉龙[2] 闫杰 李季[4] 李晓亮[4] 李先华[4] 周容 王贤芝 任志鹏 何东升 李欣[1] 潘登科 李巅远 Zhang Gen;Wang Huan;Guan Yulong;Yan Jie;Li Ji;Li Xiaoliang;Li Xianhua;Zhou Rong;Wang Xianzhi;Ren Zhipeng;He Dongsheng;Li Xin;Pan Dengke;Li Dianyuan(Department of Cardiovascular Surgery,Affiliated Suzhou Hospital of GuSu College,Nanjing Medical University,Suzhou 215000,China;Extracorporeal Circulation Center,National Cardiovascular Disease Center,Fuwai Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100037,China;Chengdu Aoge Biotechnology Co.,Ltd.,Chengdu 610094,China;Department of Thoracic and Cardiac Surgery,First People's Hospital of Neijiang,Neijiang 641000,China;Department of Ultrasound,First People's Hospital of Neijiang,Neijiang 641000,China;Department of Thoracic and Cardiac Surgery,First People's Hospital of Guangyuan,Guangyuan 628000,China;Clinical Immunology Translational Medicine Sichuan Key Laboratory,Affiliated Hospital of Electronic Science and Technology University,Sichuan Academy of Medical Sciences&Sichuan Provincial People's Hospital,Chengdu 610072,China)

机构地区：[1]南京医科大学姑苏学院附属苏州医院心血管外科,苏州215000 [2]中国医学科学院北京协和医学院国家心血管病中心阜外医院体外循环中心,北京100037 [3]成都中科奥格生物科技有限公司,成都610094 [4]内江市第一人民医院胸心外科,内江641000 [5]内江市第一人民医院超声科,内江641000 [6]广元市第一人民医院胸心外科,广元628000 [7]电子科技大学附属医院四川省医学科学院四川省人民医院临床免疫转化医学四川省重点实验室,成都610072

出　　处：《中华胸心血管外科杂志》2024年第6期379-384,共6页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：苏州市姑苏卫生人才计划(GSW2022065);苏州市临床重点病种诊疗技术专项项目(LCZX202211);南京医科大学姑苏学院引进人才项目(GSRCKY20210101)。

摘　　要：目的探讨多基因编辑猪心实施异种异位心脏移植后心功能的变化趋势,及受体免疫反应对供体心脏的影响,为基因编辑技术应用于临床领域提供实验基础。方法2023年12月16日完成猪-恒河猴异种异位心脏移植,观察移植术后负荷状态下移植物功能状态及受体免疫指标。结果受体猴已存活40天,供、受体心脏功能良好,未出现超急性和急性免疫排斥反应。结论多基因编辑技术为异种移植提供了可能,但临床应用仍需要继续深入研究。Objective To investigate the changing trends in cardiac function following xenogeneic heterotopic heart transplantation of multi-gene edited pig hearts and assess the impact of recipient immune responses on donor heart,laying experimental groundwork for the clinical application of gene editing technology.Methods On December 16,2023,xenogeneic heterotopic heart transplantation was performed between pigs and rhesus monkeys.Functional status of the graft under post-transplantation load conditions and recipient immune indicators were observed.Results The recipient monkeys survived for 40 days with satisfactory functionality of both donor and recipient hearts,and no hyperacute or acute immune rejection reactions were observed.Conclusion Multi-gene editing technology provides potential for xenotransplantation,yet further exploration is needed for its clinical application.

关 键 词：基因编辑猪 恒河猴 异种心脏移植 免疫排斥 

分 类 号：R654.2[医药卫生—外科学]