Derivation of zebrafish heart-related haploid cells  

作　　者：Siqi Liu Jia Xu Yirui Ai Yunbin Zhang Shifeng Li Jinsong Li Yiping Li 

机构地区：[1]State Key Laboratory of Cell Biology,Shanghai Key Laboratory of Molecular Andrology,CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,China

出　　处：《Journal of Molecular Cell Biology》2023年第12期65-67,共3页分子细胞生物学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(2019YFA0109900 and 2018YFA0801003);CAS Center for Excellence in Molecular Cell Science(2021DF06);Shanghai Municipal Science and Technology Major Project.

摘　　要：The first long-term cultured haploid embryonic stem cell(ESC)line was established from medaka fish,which represented a breakthrough in vertebrates(Yi et al.,2009).Subsequently,haploid ESC lines were established from mammals and used for genetic analysis and complex genetic manipulation(Elling et al.,2011;Yang et al.,2012).The diploidization of haploid cells is an interesting biological phenomenon and has been widely studied,but the mechanism of diploidization has not yet been fully resolved(Sun et al.,2020).The zebrafish is an excellent vertebrate animal model used in biological studies(Huang et al.,2022).Researchers have isolated many cell lines from zebrafish,such as the zebrafish fibroblast line ZF4(Driever and Rangini,1993).However,there have been no reports regarding stable haploid cell lines of zebrafish,and whether diploidization occurs in zebrafish is unclear.

关 键 词：FISH BREAKTHROUGH 

分 类 号：Q78[生物学—分子生物学]