龙眼叶黄酮类有效组分配伍体外降糖作用的研究  

Study on Hypoglycemic Effect of Flavonoids Active Component Compatibility from the Leaves of Dimocarpus longan Lour. in vitro

作　　者：梁晶春 胡珏 安施佳 郑飘雪 陆春莲 梁炎丽 陈明伟 梁洁 LIANG Jingchun;HU Jue;AN Shijia;ZHENG Piaoxue;LU Chunlian;LIANG Yanli;CHEN Mingwei;LIANG Jie(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi,530200,China;Key Laboratory of TCM Extraction and Purification and Quality Analysis in Guangxi Universities,Nanning,Guangxi,530200,China;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Nanning,Guangxi,530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200 [2]广西高校中药提取纯化与质量分析重点实验室,广西南宁530200 [3]广西壮瑶药重点实验室,广西南宁530200

出　　处：《广西科学院学报》2024年第2期135-144,共10页Journal of Guangxi Academy of Sciences

基　　金：国家自然科学基金项目(82160771);广西壮瑶药重点实验室项目(桂科基字[2014]32号);国家中医药管理局高水平中医药重点学科建设项目“少数民族药学(壮药学)”(zyyzdxk-2023165);广西中医药多学科交叉创新团队项目(GZKJ2309);广西高等学校千名中青年骨干教师培育计划项目(桂教人[2019]5号);壮瑶药协同创新中心项目(桂教科研[2013]20号);广西壮族自治区民族药资源与应用工程研究中心项目(桂发改高技函[2020]2605号);广西重点研发计划项目(桂科AB21196016);广西中医药重点学科壮药学项目(GZXK-Z-20-64);广西一流学科中药学(民族药学)项目(桂教科研[2018]12号);广西科技基地和人才专项(桂科AD20238058、桂科AD21238031);广西中医药大学第三批“岐黄工程”高层次人才团队培育项目(202406)资助。

摘　　要：基于体外降糖活性优选龙眼(Dimocarpus longan Lour.)叶黄酮有效成分山柰酚、槲皮素、槲皮苷的配伍比例。建立HepG2细胞胰岛素抵抗模型,分别考察不同浓度的胰岛素及不同诱导时间对胰岛素抵抗模型的诱导情况,并以山柰酚、槲皮素、槲皮苷三者用量为考察因素,以对胰岛素抵抗的HepG2细胞葡萄糖消耗率为指标,运用星点设计-效应面法(CCD-RSM)优化三者配伍比例。结果表明,建立HepG2细胞胰岛素抵抗模型的最佳条件为1×10^(-7)mol/L胰岛素诱导24 h;山柰酚、槲皮素、槲皮苷体外降糖最佳配伍比例为山柰酚105.0μmol/L、槲皮素78.9μmol/L、槲皮苷194.0μmol/L,配伍比例优化试验拟合方程为Y=20.94+1.55×A-0.44×B-2.05×C-0.66×AB+1.68×AC-1.53×A^(2)-3.2×B^(2)-2.68×C^(2)。山柰酚、槲皮素、槲皮苷3种单体的联合使用对HepG2细胞的葡萄糖消耗率预测值是21.52%,实验值是(21.05±0.52)%。优选出的最佳配伍比例的龙眼叶黄酮类有效组分,对HepG2细胞的葡萄糖消耗率最高,降糖活性较好,可为龙眼叶治疗糖尿病的有效性提供科学方法与实验依据。Based on the hypoglycemic activity in vitro,the compatibility ratio of kaempferol,quercetin and quercitrin of flavonoids from the leaves of Dimocarpus longan Lour.was optimized.The insulin resistance model of HepG2 cells was established.The induction of insulin resistance model by different concentrations of insulin and different induction time was investigated.The dosage of kaempferol,quercetin and quercitrin was used as the investigation factor,and the glucose consumption rate of insulin resistant HepG2 cells was used as the index.The star design response surface method was used to optimize the compatibility ratio of the three.The results showed that the optimal condition for establishing the insulin resistance model of HepG2 cells was 1×10^(-7) mol/L insulin induction for 24 h.The optimal compatibility ratio of kaempferol,quercetin and quercitrin in vitro was kaempferol 105.0μmol/L,quercetin 78.9μmol/L and quercitrin 194.0μmol/L.The fitting equation of compatibility ratio optimization test was Y=20.94+1.55×A-0.44×B-2.05×C-0.66×AB+1.68×AC-1.53×A^(2)-3.2×B^(2)-2.68×C^(2).The combined use of kaempferol,quercetin and quercitrin predicted a glucose consumption rate of 21.52%in HepG2 cells,and the experimental value was(21.05±0.52)%.The optimal compatibility ratio has the highest glucose consumption rate and better hypoglycemic activity for HepG2 cells,which could provide scientific methods and experimental basis for the effectiveness of D.longan Lour.leaves in the treatment of diabetes.

关 键 词：龙眼叶 组分配伍 山柰酚 槲皮素 槲皮苷 

分 类 号：R285[医药卫生—中药学]