香蕉间作绿肥对土壤氮转化及叶片酶活性的影响  

Effects of intercropping of banana with green manure crops on soil nitrogen transformation and banana leaf enzyme activity

作　　者：吕荣婷 刘新月 伍巧慧 龚文坤 王蓓蓓 LYU Rongting;LIU Xinyue;WU Qiaohui;GONG Wenkun;WANG Beibei(School of Tropical Agriculture and Forestry,Hainan University,Haikou,Hainan 570228,China)

机构地区：[1]海南大学热带农林学院,海口570228

出　　处：《热带生物学报》2024年第4期407-413,共7页Journal of Tropical Biology

基　　金：国家自然科学基金项目(42367015);海南省自然科学基金高层次人才项目(320RC483,421RC1057)。

摘　　要：为探明豆科绿肥与香蕉间作下的土壤氮转化及氮代谢的变化规律,本研究采用盆栽试验,设置单作香蕉(Mimosa nana)、间作白三叶草(Trifolium repens)、间作草木樨(Melilotus officinalis)、间作黑麦草(Lolium perenne)4个处理,测定不同时期的土壤养分含量及氮代谢关键酶的变化。结果表明:香蕉与豆科绿肥间作的土壤p H、有机质、碱解氮、全氮、有效磷和速效钾的含量均有不同程度的提高,同时也提高了土壤中铵态氮、硝态氮的含量,增加了土壤中氮素的净矿化速率和净硝化速率。与单作香蕉相比,间种豆科绿肥后,香蕉叶片的硝酸还原酶(NR)、亚硝酸还原酶(NiR)及谷氨酰酸合成酶(GS)的活性均有明显提高。土壤铵态氮、硝态氮与亚硝酸还原酶(NiR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)间表现出显著的正相关关系。An attempt was made to explore the changes of soil nitrogen transformation and nitrogen metabolism in banana plantations intercropped with leguminous green manure crops.A pot experiment was conducted to set up five treatments:monoculture of banana(CK),intercropping with Trifolium repens L(BT),intercropping with Melilotus officinalis(BM),intercropping with Lolium perenne L(BR).The changes of soil nutrient content and key enzymes for nitrogen metabolism in different sampling periods were determined.The results showed that the soil pH and the contents of organic matter,available nitrogen,total nitrogen,available phosphorus and available potassium in banana and intercrops,leguminous green manure crops,were increased in different degrees,and also increased were the soil contents of NH_(4)^(+)-N and NO_(3)^(-)-N,and the net mineralization rate and the net nitrification rate of nitrogen in the soil.After intercropping with leguminous green manure crops,the activities of nitrate reductase(NR),nitrite reductase(NiR)and glutamine synthetase(GS)in banana leaves were significantly higher than those in the monoculture banana.The soil NH_(4)^(+)-N and NO_(3)^(-)-N were significantly positively correlated with the NiR,GS and glutamate synthetase(GOGAT)in the banana leaves.

关 键 词：豆科绿肥 香蕉 间作 氮转化 酶活性 

分 类 号：S668.1[农业科学—果树学]