DNA条形码技术在快速鉴定石斛兰斑翅康瘿蚊(Contarinia maculipennis Felt)上的应用  

Application of DNA barcoding in rapid identification of Contarinia maculipennis Felt infesting Dendrobium nobile

作　　者：蔡波 赵晨阳 李芬[2] 陈施明 贾静 刘福秀 吴少英 CAI Bo;ZHAO Chenyang;LI Fen;CHEN Shiming;JIA Jing;LIU Fuxiu;WU Shaoying(Post-Entry Quarantine Station for Tropical Plant,Haikou Customs District,Haikou,Hainan 570311,China;Nanfan School(Sanya Nanfan Institute),Hainan University,Sanya,Hainan 572025,China)

机构地区：[1]海口海关热带植物隔离检疫中心,海口570311 [2]海南大学三亚南繁研究院,海南三亚572025

出　　处：《热带生物学报》2024年第4期493-498,共6页Journal of Tropical Biology

基　　金：国家重点研发计划项目(2021YFC2600600);海南省重大科技计划项目(ZDKJ2021016)。

摘　　要：石斛兰(Dendrobium nobile)因花形独特、色彩丰富,且植株具有很大药用价值,而深受国际市场喜受。石斛兰在热带、亚热带地区有广泛种植。斑翅康瘿蚊(Contarinia maculipennis Felt)的幼虫可藏于石斛兰花苞中,造成花苞畸形、变色,受到斑翅康瘿蚊危害的兰花的观赏性大大降低,影响其经济价值;斑翅康瘿蚊的老熟幼虫还可弹跳入土化蛹,随鲜花贸易进行远距离传播,目前斑翅康瘿蚊的传播已对日本的冲绳、福冈、宫崎和美国的夏威夷等地的石斛兰产业造成严重经济损失。传统瘿蚊科昆虫种类鉴定主要以形态学特征为依据,对于相近种,尤其是幼虫和卵在形态学鉴定上存在着很大局限性,故需寻找准确、快速鉴定斑翅康瘿蚊的方法,同进建议谨防其入侵我国其他的石斛兰种植区。本研究通过DNA条形码技术成功鉴定采自海南岛的样本为斑翅康瘿蚊,并详细描述了斑翅康瘿蚊的形态特征,为相关检疫部门准确、快速鉴定斑翅康瘿蚊提供科学依据。Dendrobium nobile has great medicinal value,unique flower shape and rich color,and hence is well favored in the international market and widely planted in tropical and subtropical areas.The larvae of Contarinia maculipennis Felt are hidden in the bracts of D.nobile for infestation,which can cause the bracts to be deformed and discolored,greatly reducing the ornamental value of D.nobile,and hence affecting its economic value.At the same time,the mature larvae of the pest can also drill into the soil to pupate,and can be spread for a long distance through trade of cut flowers and potted soil due to transportation.At present,this pest has caused serious economic losses to the Dendrobium industry in Okinawa,Fukuoka,Miyazaki,Hawaii and other places in Japan.The insects of family Cecidomyidae are traditionally identified mainly through morphology,which identification method is not accurate especially for the eggs and larvae of its similar species.An accurate and rapid identification of C.maculipennis Felt is hence needed to prevent its invasion into other Dendrobium nobile plantation areas in China.DNA barcoding technology was used to successfully identify the samples collected from a China(Sanya)International Orchid Show,Hainan Island as C.maculates Felt,and the morphological characteristics of C.maculates Felt were described in detail,which provided a scientific basis for accurate and rapid identification of C.maculates Felt by relevant quarantine departments.

关 键 词：石斛兰 斑翅康瘿蚊 形态特征 分子鉴定 

分 类 号：S41-30[农业科学—植物保护]